AJP - GI Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 273: G833-G841, 1997;
0193-1857/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hochman, J. A.
Right arrow Articles by Cohen, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hochman, J. A.
Right arrow Articles by Cohen, M. B.
Vol. 273, Issue 4, G833-G841, October 1997

Hepatocyte nuclear factor-1alpha regulates transcription of the guanylin gene

J. A. Hochman, D. Sciaky, T. L. Whitaker, J. A. Hawkins, and M. B. Cohen

Division of Pediatric Gastroenterology and Nutrition, Children's Hospital Medical Center and the University of Cincinnati, Cincinnati, Ohio 45229

To study the molecular mechanisms controlling guanylin expression, we have cloned the mouse guanylin gene, including 2.7 kb of upstream sequence. We show that the first 133 base pairs (bp) of the upstream guanylin promoter are sufficient to drive near maximal (6-fold over basal) luciferase reporter gene expression in Caco-2 intestinal cells; at least 300 bp of upstream promoter are required for reporter gene expression in HT-29 intestinal cell lines. Using electromobility shift assays, we demonstrate that nuclear proteins bind to the hepatocyte nuclear factor-1 (HNF-1) consensus sequence in the guanylin promoter. The HNF-1 consensus sequence, located in the immediate 5' flanking region, is required for transcriptional activation of the guanylin gene in both intestinal cell lines. Mutagenesis of the HNF-1 consensus sequence abolishes transcriptional activation of guanylin promoter-luciferase reporter gene constructs. Cotransfection of these constructs with HNF-1alpha augments transcriptional initiation of the reporter gene. In contrast, HNF-1beta has no significant effect on transcription of the reporter gene. These experiments demonstrate that HNF-1alpha is an important regulatory element in the transcriptional activation of guanylin.

Caco-2 cells; HT-29 cells; guanylate cyclase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Bosse, H. M. van Wering, M. Gielen, L. N. Dowling, J. J. Fialkovich, C. M. Piaseckyj, F. J. Gonzalez, T. E. Akiyama, R. K. Montgomery, R. J. Grand, et al.
Hepatocyte nuclear factor-1{alpha} is required for expression but dispensable for histone acetylation of the lactase-phlorizin hydrolase gene in vivo
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G1016 - G1024.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Olsen, S. Bressendorff, J. T. Troelsen, and J. Olsen
Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G220 - G226.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. K. Divine, S. P. McCaul, and T. C. Simon
HNF-1{alpha} and endodermal transcription factors cooperatively activate Fabpl: MODY3 mutations abrogate cooperativity
Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G62 - G72.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. A. Steinbrecher, J. A. Rudolph, G. Luo, and M. B. Cohen
Coordinate upregulation of guanylin and uroguanylin expression by hypertonicity in HT29-18-N2 cells
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1729 - C1737.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. A. Rudolph, J. A. Hawkins, and M. B. Cohen
Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G695 - G702.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. D. Krasinski, H. M. Van Wering, M. R. Tannemaat, and R. J. Grand
Differential activation of intestinal gene promoters: functional interactions between GATA-5 and HNF-1{alpha}
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G69 - G84.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. A. Scheving and W.-H. Jin
Circadian regulation of uroguanylin and guanylin in the rat intestine
Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1177 - C1183.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Zahedi, J. J. Bissler, A. E. Prada, J. A. Prada, and A. E. Davis III
The Promoter of the C1 Inhibitor Gene Contains a Polypurine{middle dot}Polypyrimidine Segment that Enhances Transcriptional Activity
J. Immunol., June 15, 1999; 162(12): 7249 - 7255.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bonham, S. A. Ritchie, S. M. Dehm, K. Snyder, and F. M. Boyd
An Alternative, Human SRC Promoter and Its Regulation by Hepatic Nuclear Factor-1alpha
J. Biol. Chem., November 22, 2000; 275(48): 37604 - 37611.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online