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Departments of 1 Internal Medicine, 2 Obstetrics and Gynecology, and 3 Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan 48109-0586
Insulin-like
growth factor (IGF) binding protein 5 (IGFBP-5) mRNA was studied in
intestines of rats with peptidoglycan-polysaccharide enterocolitis by
Northern analysis and in situ hybridization. IGFBP-5
mRNA was increased 2.4 ± 0.5-fold in inflamed rat colon compared
with controls and was highly expressed in smooth muscle. Cultured rat
intestinal smooth muscle cells were used to study the regulation of
IGFBP-5 and type I collagen synthesis. IGF-I (100 ng/ml) increased
IGFBP-5 mRNA (1.9 ± 0.1-fold) and collagen type
1(I) mRNA (1.6 ± 0.2-fold)
in cultured smooth muscle cells. IGF-I induced a dose- and
time-dependent increase in IGFBP-5 in conditioned medium by Western
ligand blot and by immunoblot. IGF-I did not affect the IGFBP-5
mRNA decay rate after transcriptional blockade. Cycloheximide abolished
IGFBP-5 mRNA. In conclusion, IGFBP-5 mRNA is expressed by
intestinal smooth muscle and is increased during chronic
inflammation. IGF-I increases IGFBP-5 and collagen mRNAs in
intestinal smooth muscle cells.
inflammatory bowel disease; Crohn's disease; intestinal fibrosis; growth factors; insulin-like growth factor I; insulin-like growth factor binding protein 5
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