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channel in guinea pig
small intestinal villus enterocytes: effect of
inhibitors
1 Department of Child Health,
Ninewells Hospital and Medical School, University of Dundee, Dundee DD1
9SY, United Kingdom; 2 Departament
de Ciències Mèdiques Bàsiques, Facultat de Medicina,
Universitat de Lleida, 25198 Lleida, Spain;
3 Departamento de Medicina
Experimental,
Previous studies in enterocytes isolated from
the villus region of small intestinal epithelium have identified a
macroscopic current carried by
Cl
. In this work a
single-channel patch-clamp study was carried out in the same cells, and
a spontaneously active, outwardly rectifying Cl channel was identified
and proposed to underlie the whole cell current. The channel had
conductances of 62 and 19 pS at 80 and 
80 mV, respectively, in
symmetrical Cl solutions in
excised patches. Similar activity was seen in cell-attached patches,
but only outward currents could be discerned in this configuration. The
activity of the channel, measured as open probability, was independent
of intracellular calcium levels and voltage. The selectivity sequence
for different anions was
SCN > I > Br > Cl > F > (gluconate,
glutamate, 
). The channel was
inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid
(NPPB), verapamil, and 4-hydroxytamoxifen (but not by
tamoxifen), with potencies similar to those observed for
Cl channels previously
described in other cells. Inhibition by trinitrophenyladenosine 5'-triphosphate was also observed but only at
depolarized potentials. At 50 mV the half-maximal inhibitory
concentration was 18 nM. It is proposed that this channel plays a role
in transepithelial Cl
transport and certain regulatory
Cl fluxes.
epithelium; chloride transport; trinitrophenyladenosine 5'-triphosphate
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