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Am J Physiol Gastrointest Liver Physiol 274: G122-G130, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 1, G122-G130, January 1998

Identification and localization of extracellular Ca2+-sensing receptor in rat intestine

Naibedya Chattopadhyay1, Ivan Cheng1, Kimberly Rogers2, Daniela Riccardi3, Amy Hall3, Ruben Diaz1, Steven C. Hebert3, David I. Soybel4,5, and Edward M. Brown1

1 Endocrine-Hypertension Division, Departments of Medicine and 4 Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston 02115; and 5 Department of Surgery, Veterans Affairs Medical Center, West Roxbury, Massachusetts 02132; 2 NPS Pharmaceuticals, Inc., Salt Lake City, Utah 84108; and 3 Nephrology Division, Vanderbilt University Medical Center, Nashville, Tennessee 37232

The extracellular calcium (Ca2+o)-sensing receptor (CaR) plays vital roles in Ca2+o homeostasis, but no data are available on its expression in small and large intestine. Polymerase chain reaction products amplified from reverse-transcribed duodenal RNA using CaR-specific primers showed >99% homology with the rat kidney CaR. Northern analysis with a CaR-specific cRNA probe demonstrated 4.1- and 7.5-kb transcripts in all intestinal segments. Immunohistochemistry with CaR-specific antisera showed clear basal staining of epithelial cells of small intestinal villi and crypts and modest apical staining of the former, whereas there was both basal and apical staining of colonic crypt epithelial cells. In situ hybridization and immunohistochemistry also demonstrated CaR expression in Auerbach's myenteric plexus of small and large intestines and in the submucosa in the region of Meissner's plexus. Our results reveal CaR expression in several cell types of small and large intestine, in which it may modulate absorptive and/or secretomotor functions.

villi; crypts; nerve plexus; Northern analysis; immunohistochemistry; in situ hybridization histochemistry


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