| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Biochemistry, 2 Chemistry, 5 Physiology, and 7 Internal Medicine, Kitasato University School of Medicine, Sagamihara 228; 4 Isehara Research Laboratory, Kanto Chemical Company, Inc., Isehara 259-11; and Departments of 3 Anatomy and 6 Physiology, Yokohama City University School of Medicine, Yokohama 236, Japan
Although gastrin, histamine, and carbachol (CCh) accelerate gastric mucin metabolism, information about their target cells of mucin production is lacking. To clarify this, we examined the effects of these stimulants, including the possible participation of nitric oxide (NO), on mucin biosynthesis in distinct sites and layers of rat gastric mucosa. Pieces of tissue obtained from the corpus and antrum were incubated in a medium containing radioactive precursors and each stimulant, with or without NO synthase (NOS) inhibitor. Distribution of NOS was compared with that of the specific mucins by immunostaining using specific antiserum and monoclonal antibodies. In the full-thickness corpus mucosa, tetragastrin enhanced [3H]glucosamine incorporation into mucin but had no effect on [14C]threonine incorporation. Both histamine and CCh dose dependently increased 3H- and 14C-labeled corpus mucin. Only CCh stimulated antral mucin biosynthesis. CCh stimulation was noted in the corpus mucosa after removal of surface mucous cells, but stimulation by tetragastrin or histamine disappeared as a result of this pretreatment. Only tetragastrin-induced activation was completely blocked by the NOS inhibitor. NOS immunoreactivity was limited to surface mucous cells. Mucus-producing cells present in the different sites and layers of the gastric mucosa have distinct mechanisms for regulation of mucin biosynthesis. Gastrin-stimulated mucin biosynthesis mediated by NO is limited to surface mucous cells of rat gastric oxyntic mucosa.
gastrin; nitric oxide; surface mucous cells; carbachol
This article has been cited by other articles:
|
|
 |
|
  C. Atuma, V. Strugala, A. Allen, and L. Holm The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G922 - G929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Premaratne, C. Xue, J. M. McCarty, M. Zaki, R. W. McCuen, R. A. Johns, W. Schepp, B. Neu, R. Lippman, P. D. Melone, et al. Neuronal nitric oxide synthase: expression in rat parietal cells Am J Physiol Gastrointest Liver Physiol, February 1, 2001; 280(2): G308 - G313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. García–Vitoria, C. García–Corchón, J. A. Rodríguez, F. García–Amigot, and M. A. Burrell Expression of Neuronal Nitric Oxide Synthase in Several Cell Types of the Rat Gastric Epithelium J. Histochem. Cytochem., August 1, 2000; 48(8): 1111 - 1120. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ichikawa, K. Ishihara, T. Kusakabe, H. Hiruma, T. Kawakami, and K. Hotta CGRP modulates mucin synthesis in surface mucus cells of rat gastric oxyntic mucosa Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G82 - G89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Konda, H. Kamimura, H. Yokota, N. Hayashi, K. Sugano, and T. Takeuchi Gastrin stimulates the growth of gastric pit with less-differentiated features Am J Physiol Gastrointest Liver Physiol, October 1, 1999; 277(4): G773 - G784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kamimura, Y. Konda, H. Yokota, S.-I. Takenoshita, Y. Nagamachi, H. Kuwano, and T. Takeuchi Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G183 - G190. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
