| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Internal Medicine, Saga Medical School, Nabeshima, Saga 849, Japan; and 2 Department of Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130
Apoptosis after ischemia-reperfusion
(I/R) was characterized in rat small intestine. Under halothane
anesthesia, the superior mesenteric artery in the rat was occluded for
15 or 60 min, followed by reperfusion. Ratios of fragmented DNA to
total DNA, electrophoresis, and immunohistochemical staining were
examined after I/R. Some rats were pretreated with
-difluoromethylornithine (DFMO) to examine the relationship between
intestinal apoptosis and ornithine decarboxylase (ODC) activity. The
percentage of fragmented DNA significantly increased just after
ischemia and peaked at 1 h after reperfusion in the jejunum and
ileum. These increases were significantly higher in the 60-min
ischemia group compared with the 15-min ischemia group.
This increase decreased 6 h after reperfusion. The results were
corroborated by histological evaluations of the intestine under the
same conditions. DFMO completely abolished elevation of ODC activity 6 h after reperfusion but did not change the percentage of fragmented
DNA. Apoptosis in rat small intestine was induced by ischemia
of the gut, and this process was exacerbated by reperfusion. The
changes in apoptosis were independent of ODC activity.
percent fragmented DNA; DNA ladder; terminal deoxynucleotidyl transferase-mediated dUDP-biotin nick end labeling; ornithine decarboxylase
This article has been cited by other articles:
|
|
 |
|
  T. Fujise, R. Iwakiri, T. Kakimoto, R. Shiraishi, Y. Sakata, B. Wu, S. Tsunada, A. Ootani, and K. Fujimoto Long-term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/beta-catenin signaling in rats Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1150 - G1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Kurtz, S. L. Lindell, M. J. Mangino, and H. V. Carey Hibernation confers resistance to intestinal ischemia-reperfusion injury Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G895 - G901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujise, R. Iwakiri, B. Wu, S. Amemori, T. Kakimoto, F. Yokoyama, Y. Sakata, S. Tsunada, and K. Fujimoto Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G110 - G116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Fleck and H. V. Carey Modulation of apoptotic pathways in intestinal mucosa during hibernation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R586 - R595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hoffman-Goetz, J. Quadrilatero, J. Boudreau, and J. Guan Adrenalectomy in mice does not prevent loss of intestinal lymphocytes after exercise J Appl Physiol, June 1, 2004; 96(6): 2073 - 2081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wu, A. Ootani, R. Iwakiri, T. Fujise, S. Tsunada, S. Toda, and K. Fujimoto Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G580 - G587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Genesca, A. Sola, R. Miquel, F. Pi, C. Xaus, V. Alfaro, and G. Hotter Role of Changes in Tissular Nucleotides on the Development of Apoptosis during Ischemia/Reperfusion in Rat Small Bowel Am. J. Pathol., November 1, 2002; 161(5): 1839 - 1847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Spies, V. L. Chappell, M. R. Dasu, D. N. Herndon, J. C. Thompson, and S. E. Wolf Role of TNF-alpha in gut mucosal changes after severe burn Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G703 - G708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Watts, R. M. Grattan II, B. S. Whitlow, and J. A. Kline Activation of poly(ADP-ribose) polymerase in severe hemorrhagic shock and resuscitation Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G498 - G506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Jeschke, J. F. A. Low, M. Spies, R. Vita, H. K. Hawkins, D. N. Herndon, and R. E. Barrow Cell proliferation, apoptosis, NF-{kappa}B expression, enzyme, protein, and weight changes in livers of burned rats Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1314 - G1320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Scheving, J. R. Thomas, and L. Zhang Regulation of intestinal tyrosine phosphorylation and programmed cell death by peroxovanadate Am J Physiol Cell Physiol, September 1, 1999; 277(3): C572 - C579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Coopersmith, D. O'Donnell, and J. I. Gordon Bcl-2 inhibits ischemia-reperfusion-induced apoptosis in the intestinal epithelium of transgenic mice Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G677 - G686. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
