AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 274: G465-G471, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wada, K.
Right arrow Articles by Itoh, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wada, K.
Right arrow Articles by Itoh, T.
Vol. 274, Issue 3, G465-G471, March 1998

Direct measurement of nitric oxide release in gastric mucosa during ischemia-reperfusion in rats

Kouichirou Wada, Yoshinori Kamisaki, Tsuyoshi Ohkura, Gaku Kanda, Kentaro Nakamoto, Yosuke Kishimoto, Kumiyo Ashida, and Tadao Itoh

Department of Clinical Pharmacology, Faculty of Medicine, Tottori University, Yonago 683, Japan

Nitric oxide (NO) generation in the rat gastric mucosa during ischemia-reperfusion was measured using an NO-sensitive electrode. Under pentobarbital sodium anesthesia, an electrode was inserted into the submucosa from the serous membrane side in the fundus. After steady-state baseline recording, the celiac artery was clamped for 30 min, and then ischemia-reperfusion was achieved by removing the clamp. The clamping of the celiac artery caused a decrease in blood flow and an increase in NO level in the gastric tissue. Just after the removal of the clamp, the NO level rapidly fell and returned to the baseline level. Administration of NG-nitro-L-arginine methyl ester (an NO synthase inhibitor, 30 mg/kg ip) before ischemia significantly attenuated both the increase in NO level during ischemia and the formation of acute gastric mucosal lesions observed after 60 min reperfusion. Administration of superoxide dismutase (a superoxide radical scavenger, 10,000 U/kg iv) at the end of ischemia inhibited both the rapid decrease in NO level during the reperfusion and the gastric mucosal erosions. Because NO and superoxide radical produce a highly reactive peroxynitrite, it can be argued that NO has an important pathological role in acute gastric mucosal injury induced by ischemia-reperfusion. Our conclusion was strongly supported by immunohistochemical staining of nitrotyrosine residues, an indication of peroxynitrite formation.

gastric mucosal erosion; superoxide radical; peroxynitrite


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. Wada, A. Nakajima, H. Takahashi, M. Yoneda, N. Fujisawa, E. Ohsawa, T. Kadowaki, N. Kubota, Y. Terauchi, N. Matsuhashi, et al.
Protective effect of endogenous PPAR{gamma} against acute gastric mucosal lesions associated with ischemia-reperfusion
Am J Physiol Gastrointest Liver Physiol, August 1, 2004; 287(2): G452 - G458.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. C. Montalto, M. L. Hart, J. E. Jordan, K. Wada, and G. L. Stahl
Role for complement in mediating intestinal nitric oxide synthase-2 and superoxide dismutase expression
Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G197 - G206.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. Iturriaga, S. Villanueva, and M. Mosqueira
Dual effects of nitric oxide on cat carotid body chemoreception
J Appl Physiol, September 1, 2000; 89(3): 1005 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Tsuji, S. Shioya, Y. Hirota, N. Fukuyama, D. Kurita, T. Tanigaki, Y. Ohta, and H. Nakazawa
Increased production of nitrotyrosine in lung tissue of rats with radiation-induced acute lung injury
Am J Physiol Lung Cell Mol Physiol, April 1, 2000; 278(4): L719 - L725.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online