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Am J Physiol Gastrointest Liver Physiol 274: G518-G524, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 3, G518-G524, March 1998

Nonionic diffusion of short-chain fatty acids across rat colon

Alan N. Charney, Ljubisa Micic, and Richard W. Egnor

Nephrology Section, Veterans Affairs Medical Center, New York University School of Medicine, New York, New York 10010

Short-chain fatty acid (SCFA) transport across the colon may occur by nonionic diffusion and/or via apical membrane SCFA-/HCO<SUP>−</SUP><SUB>3</SUB> exchange. To examine the relative importance of these processes, stripped segments of rat (Ratus ratus) proximal and distal colon were studied in Ussing chambers, and the unidirectional fluxes of radiolabeled SCFA butyrate, propionate, or weakly metabolized isobutyrate were measured. In N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) or 1 or 5 mM HCO<SUP>−</SUP><SUB>3</SUB> Ringer, decreases in mucosal pH stimulated mucosal-to-serosal flux (Jmright-arrow s) of all SCFA, decreases in serosal pH stimulated serosal-to-mucosal flux (Jsright-arrow m), and bilateral pH decreases stimulated both fluxes equally. These effects were observed whether the SCFA was present on one or both sides of the tissue, in both proximal and distal colon, in the absence of luminal Na+, and in the presence of either luminal or serosal ouabain. Changes in intracellular pH or intracellular [HCO<SUP>−</SUP><SUB>3</SUB>] did not account for the effects of extracellular pH. Luminal Cl- removal, to evaluate the role of apical membrane Cl-/SCFA- exchange, had no effect on Jmright-arrow s but decreased Jsright-arrow m 32% at pH 6.5 and 22% at 7.2. Increasing SCFA concentration from 1 to 100 mM, at pH 6.4 or 7.4, caused a linear increase in Jmright-arrow s. We conclude that SCFA are mainly transported across the rat colon by nonionic diffusion.

butyrate; propionate; in vitro; flux; pH; intracellular pH


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