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Am J Physiol Gastrointest Liver Physiol 274: G871-G878, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 5, G871-G878, May 1998

MUC5B is the prominent mucin in human gallbladder and is also expressed in a subset of colonic goblet cells

B. Jan-Willem Van Klinken1, Jan Dekker2, Sandy A. Van Gool1, Jan Van Marle3, Hans A. Büller2, and Alexandra W. C. Einerhand2

1 Pediatric Gastroenterology and Nutrition and 3 Department of Electron Microscopy, Academic Medical Center, and Department of Pediatrics, Emma Children's Hospital AMC, University of Amsterdam, 1105 AZ Amsterdam; and 2 Department of Pediatrics, Sophia Children's Hospital, 3015 GJ Rotterdam, The Netherlands

To elucidate the roles of human gallbladder mucin (HGBM), such as in gallstone formation and cytoprotection, it is essential to identify HGBM and study its expression. This was performed by metabolic labeling, Western blotting, immunohistochemistry, and RT-PCR. In a large number of individuals, antibodies against purified HGBM and against MUC5B detected a mucin precursor (~470 kDa) in the gallbladder and colon, but not in the small intestine. In the gallbladder, Western blotting using specific anti-MUC5B antibodies showed that this mucin precursor represented an identical mucin, MUC5B. RT-PCR experiments demonstrated a similar tissue distribution pattern of MUC5B mRNA. Immunohistochemistry with anti-HGBM and anti-MUC5B showed staining in gallbladder epithelial cells and colonic goblet cells in the crypt base, but not in the small intestine; double labeling showed that HGBM was located in small granules within goblet cells, colocalizing to MUC2-containing goblet cells. Metabolic labeling demonstrated the secretion of mature MUC5B in the colon. Conclusively, MUC5B is identified as the prominent HGBM and is also expressed and secreted in the colon.

biosynthesis; colon


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