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Am J Physiol Gastrointest Liver Physiol 274: G1045-G1052, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 6, G1045-G1052, June 1998

Electrogenic bicarbonate secretion in mouse gallbladder

L. C. Martin, M. E. Hickman, C. M. Curtis, L. J. MacVinish, and A. W. Cuthbert

Department of Pharmacology, University of Cambridge, Cambridge CB2 1QJ, United Kingdom

Mouse gallbladders (4 mm2) were investigated using the short-circuit current (Isc) technique. Responses of 50 µA/cm2 were obtained in response to forskolin and agents that stimulated the adenylate cyclase system (IBMX and dibutyryl-cAMP). The calcium ionophore ionomycin increased Isc to 30% of the forskolin-stimulated increase. The forskolin-dependent current was inhibited 40% by acetazolamide but was insensitive to furosemide. Forskolin responses were dependent on the presence of bicarbonate ions; removal from both sides of the membrane or the basolateral side alone caused a significant reduction in responses. Removal of chloride ions from the basolateral side had no effect, while removal from the apical side caused a significant reduction in the forskolin responses, but only by 30%. It is argued that the remaining current (70%) cannot result from a parallel arrangement of a chloride channel and a chloride-bicarbonate exchanger and that bicarbonate is secreted through the apical membrane by a predominantly conductive mechanism. Apparently, forskolin converts a near electrically silent epithelium to an electrogenically secreting tissue.

electrogenic transport; forskolin; acetazolamide


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