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Am J Physiol Gastrointest Liver Physiol 274: G1077-G1086, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 6, G1077-G1086, June 1998

Pancreatic trypsinogen I expression during cell growth and differentiation of two human colon carcinoma cells

Françoise Bernard-Perrone1, Jacqueline Carrere2, Wanda Renaud3, Christine Moriscot3, Karine Thoreux1, Patrice Bernard1, Alain Servin4, Daniel Balas1, and Françoise Senegas-Balas1

1 Groupe de Recherche sur la Trophicité et le Vieillissement, Faculté de Médecine, 06107 Nice Cedex 2; 3 Groupe de Recherche sur les Glandes Exocrines, 13385 Marseille Cedex; 2 Hopital Renée Sabran, Giens, 83406 Hyères Cedex; and 4 Contrat Jeune Formation, Institut National de la Santé et de la Recherche Médicale 94-07, Unité de Formation et de Recherche-Sciences-Pharmacie, Paris XI, 92296 Chatenay-Malabry Cedex, France

Pancreatic trypsin has been found to induce tight junction or dome formation in some colon cancer cell lines (HT-29, Caco-2), and a tumor-associated trypsinogen, trypsinogen type II, has been isolated from another colon cancer cell line (COLO 205). We have tried to determine if trypsinogen is present and how its expression varies during cell culture in HT-29 Glc+/- and Caco-2 cells, which exhibit enterocytic differentiation, and in HT-29 Glc+ cells, which never differentiate. Trypsinogen mRNA presence and expression were demonstrated in these cells by mRNA hybridization, RT-PCR, cytoimmunofluorescence, Western immunoblot analysis, and gel filtration. Trypsinogen was found to be trypsinogen type I and was mainly in zymogen form in culture media. Differentiating cells exhibited variations in trypsinogen I expression, but cells that remained undifferentiated did not. In the differentiated cells, a high and transient peak in trypsinogen I expression was observed during the first steps of differentiation.

HT-29; Caco-2


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