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Am J Physiol Gastrointest Liver Physiol 274: G1094-G1100, 1998;
0193-1857/98 $5.00
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Vol. 274, Issue 6, G1094-G1100, June 1998

Colonic mucin release in response to immobilization stress is mast cell dependent

Ignazio Castagliuolo1, Barry K. Wershil2,3, Katia Karalis1,4, Asiya Pasha1, Sigfus T. Nikulasson5, and Charalabos Pothoulakis1

Divisions of 1 Gastroenterology and 2 Experimental Pathology, Beth Israel Deaconess Medical Center, 3 The Combined Program in Pediatric Gastroenterology and Nutrition, and 4 Division of Endocrinology, Children's Hospital, Harvard Medical School, Boston 02215; and 5 Department of Pathology, Boston University School of Medicine, Boston, Massachusetts 02118

We recently reported that immobilization stress increased colonic motility, mucin, and prostaglandin E2 (PGE2) release and mucosal mast cell degranulation in rat colon [Proc. Natl. Acad. Sci. USA 93: 12611-12615, 1996; Am. J. Physiol. 271 (Gastrointest. Liver Physiol. 34): G884-G892, 1996]. To directly assess the contribution of mast cells, we compared colonic responses to stress in mast cell-deficient KitW/KitW-v and normal (+/+) mice. Mucin and PGE2 release were measured in colonic explants cultured from KitW/KitW-v and (+/+) mice 30 min after immobilization stress. We found that stress stimulated colonic mucin release (1.8-fold), goblet cell depletion (3-fold), and PGE2 (2.3-fold) release in (+/+) but not mast cell-deficient KitW/KitW-v mice. However, mast cell-deficient mice that had their mast cell population reconstituted by injection of bone marrow-derived mast cells from (+/+) mice had colonic responses to stress similar to those of normal (+/+) mice. In contrast, colonic transit changes in response to stress, estimated by fecal output, were similar between KitW/KitW-v and normal (+/+) mice. We conclude that mast cells regulate colonic mucin and PGE2 release but not colonic transit changes in response to immobilization stress.

mast cell-deficient mice; prostaglandin E2; colonic motility; hypothalamic-pituitary-adrenal axis; central nervous system


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