AJP - GI AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 275: G203-G211, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, P.
Right arrow Articles by Behar, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, P.
Right arrow Articles by Behar, J.
Vol. 275, Issue 2, G203-G211, August 1998

Signal transduction pathways mediating CCK-induced gallbladder muscle contraction

Peirong Yu, Qian Chen, Zuoliang Xiao, Karen Harnett, Piero Biancani, and Jose Behar

Department of Medicine, Rhode Island Hospital, and Brown University School of Medicine, Providence, Rhode Island 02903

The signal transduction that mediates CCK-induced contraction of gallbladder muscle was investigated in the cat. Contraction was measured by scanning micrometry in single muscle cells isolated enzymatically with collagenase. Production of D-myo-inositol 1,4,5-trisphosphate (IP3) and sn-1,2-diacylglycerol (DAG) was quantitated using HPLC and TLC, respectively. Protein kinase C (PKC) activity was determined by measuring the phosphorylation of a specific substrate peptide from myelin basic protein, Ac-MBP-(4---14). CCK-induced contraction was blocked by incubation in strontium medium, pertussis toxin (PTx), and antibodies against Gialpha 3 or beta gamma -subunits but was not blocked by Ca2+-free medium or by antibodies against Gq/11alpha , Gialpha 1-2, or Goalpha . The contraction induced by CCK was inhibited by the phospholipase C (PLC) inhibitor U-73122, anti-PLC-beta 3 antibody, and the IP3 receptor antagonist heparin but was not inhibited by the the phospholipase D inhibitor propranolol or antibodies against PLC-beta 1 or PLC-beta 2. Western blot analysis of gallbladder muscle revealed the presence of PLC-beta 2 and PLC-beta 3 but not PLC-beta 1. CCK caused a 94% increase in IP3 generation and an 86% increase in DAG generation. A low dose of CCK caused PKC translocation, and CCK-induced contraction was blocked by the PKC inhibitor H-7. A high dose of CCK, however, caused no PKC translocation, and its contraction was blocked by the calmodulin antagonist CGS9343B. In conclusion, CCK contracts cat gallbladder muscle by stimulating PTx-sensitive Gi 3 protein coupled with PLC-beta 3, producing IP3 and DAG. Low doses activate PKC, whereas high doses activate calmodulin.

G proteins; phospholipase C; inositol 1,4,5-trisphosphate; protein kinase C


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. B. Balemba, T. J. Heppner, A. D. Bonev, M. T. Nelson, and G. M. Mawe
Calcium waves in intact guinea pig gallbladder smooth muscle cells
Am J Physiol Gastrointest Liver Physiol, October 1, 2006; 291(4): G717 - G727.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Dufresne, C. Seva, and D. Fourmy
Cholecystokinin and gastrin receptors.
Physiol Rev, July 1, 2006; 86(3): 805 - 847.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Z.-L. Xiao, W. Cao, P. Biancani, and J. Behar
Nongenomic effects of progesterone on the contraction of muscle cells from the guinea pig colon
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): 1008 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. B. Balemba, M. J. Salter, T. J. Heppner, A. D. Bonev, M. T. Nelson, and G. M. Mawe
Spontaneous electrical rhythmicity and the role of the sarcoplasmic reticulum in the excitability of guinea pig gallbladder smooth muscle cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G655 - G664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Morales, P. J. Camello, G. M. Mawe, and M. J. Pozo
Characterization of intracellular Ca2+ stores in gallbladder smooth muscle
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G507 - G513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Morales, P. J. Camello, S. Alcon, G. M. Salido, G. Mawe, and M. J. Pozo
Coactivation of capacitative calcium entry and L-type calcium channels in guinea pig gallbladder
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G1090 - G1100.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. P. L. Guarino, Z.-L. Xiao, P. Biancani, and J. Behar
PAF-like lipids- and PAF-induced gallbladder muscle contraction is mediated by different pathways in guinea pigs
Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1189 - G1197.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. K. Sarna
Neuronal locus and cellular signaling for stimulation of ileal giant migrating and phasic contractions
Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G789 - G797.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. Y. Shin, Y. P. Lee, T. S. Lee, H. D. Je, D. S. Kim, and U. D. Sohn
The Signal Transduction of Endothelin-1-Induced Circular Smooth Muscle Cell Contraction in Cat Esophagus
J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 924 - 934.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. Piiper, R. Gebhardt, B. Kronenberger, C. D. Giannini, R. Elez, and S. Zeuzem
Pertussis Toxin Inhibits Cholecystokinin- and Epidermal Growth Factor-Induced Mitogen-Activated Protein Kinase Activation by Disinhibition of the cAMP Signaling Pathway and Inhibition of c-Raf-1
Mol. Pharmacol., September 1, 2000; 58(3): 608 - 613.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online