AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 275: G542-G549, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qu, W.
Right arrow Articles by Thurman, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qu, W.
Right arrow Articles by Thurman, R. G.
Vol. 275, Issue 3, G542-G549, September 1998

Stimulation of oxygen uptake by prostaglandin E2 is oxygen dependent in perfused rat liver

Wei Qu, Zhi Zhong, Gavin E. Arteel, and Ronald G. Thurman

Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599-7365

The aim of this study was to determine if the effect of prostaglandin E2 (PGE2) on hepatic oxygen uptake was affected by oxygen tension. Livers from fed female Sprague-Dawley rats were perfused at normal or high flow rates (4 or 8 ml · g-1 · min-1) to vary local oxygen tension within the liver lobule. During perfusion at normal flow rates, PGE2 (5 µM) infusion increased oxygen uptake by about 50 µmol · g-1 · h-1; however, when livers were perfused at high flow rates, the increase was nearly twice as large. Simultaneously, glucose output was increased rapidly by about 50%, whereas glycolysis was decreased about 60%. When flow rate was held constant, increases in oxygen uptake due to PGE2 were proportional to oxygen delivery. Infusion of PGE2 into livers perfused at normal flow rates increased state 3 rates of oxygen uptake of subsequently isolated mitochondria by about 25%; however, rates were increased 50-75% in mitochondria isolated from livers perfused at high flow rates. Thus it is concluded that PGE2 stimulates oxygen uptake via mechanisms regulated by oxygen tension in perfused rat liver. High flow rates also increased basal rates of oxygen uptake: this increase was prevented by inactivation of Kupffer cells with GdCl3. In addition, conditioned medium from Kupffer cells incubated at high oxygen tension (75% oxygen) stimulated oxygen uptake of isolated parenchymal cells by >30% and elevated PGE2 production about twofold compared with Kupffer cells exposed to normal air-saturated buffer (21% oxygen). These effects were blocked completely by both indomethacin and nisoldipine. These data support the hypothesis that oxygen stimulates Kupffer cells to release mediators such as PGE2 which elevate oxygen consumption in parenchymal cells, possibly by mechanisms involving cyclooxygenase and calcium channels.

Kupffer cells; eicosanoids; hypermetabolic state


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Yokoyama, H. Xu, N. Kresge, S. Keller, A. H. Sarmadi, R. Baveja, M. G. Clemens, and J. X. Zhang
Role of thromboxane A2 in early BDL-induced portal hypertension
Am J Physiol Gastrointest Liver Physiol, March 1, 2003; 284(3): G453 - G460.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. Qu, K. Ikejima, Z. Zhong, M. P. Waalkes, and R. G. Thurman
Glycine blocks the increase in intracellular free Ca2+ due to vasoactive mediators in hepatic parenchymal cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1249 - G1256.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. H. Goldbarg, Y. Takahashi, C. Cruz, H. Kajino, C. Roman, B. M. Liu, Y. Q. Chen, F. Mauray, and R. I. Clyman
In utero indomethacin alters O2 delivery to the fetal ductus arteriosus: implications for postnatal patency
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R184 - R190.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. Qu, L. M. Graves, and R. G. Thurman
PGE2 stimulates O2 uptake in hepatic parenchymal cells: involvement of the cAMP-dependent protein kinase
Am J Physiol Gastrointest Liver Physiol, November 1, 1999; 277(5): G1048 - G1054.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online