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Am J Physiol Gastrointest Liver Physiol 275: G584-G591, 1998;
0193-1857/98 $5.00
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Vol. 275, Issue 3, G584-G591, September 1998

Migration of intestinal intraepithelial lymphocytes into a polarized epithelial monolayer

Sunil K. Shaw1, Anne Hermanowski-Vosatka1, Takeshi Shibahara2, Beth A. McCormick1, Charles A. Parkos1, Susan L. Carlson1, Ellen C. Ebert3, Michael B. Brenner4, and James L. Madara1

1 Division of Gastrointestinal Pathology, Department of Pathology, and 4 Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, Massachusetts 02115; 3 Department of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903; and 2 Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322

Intraepithelial lymphocytes (IEL) are a phenotypically distinct population of lymphocytes that reside in mucosal epithelia, below the intercellular tight junctions. Although adhesive functions of this population have been previously studied, relatively little is known about IEL migration from the microvasculature into the epithelium. We demonstrated that cultured human IEL were capable of migration into polarized epithelial cells in vitro, where they assumed a subjunctional position, identical to that observed in vivo. The migration was rapid and efficient and was directionally polarized, such that IEL migrated into epithelial monolayers from the basolateral, but not the apical, aspect. After a 4-h period of residence, up to one-half of the IEL then exited the monolayer basolaterally. Migration was partially inhibited by pertussis toxin, suggesting a potential mechanism for IEL migration by chemokine receptor-mediated signaling. The conditions and ligand pairs used in IEL migration were different from those for neutrophils, another cell type known to migrate through epithelia. This system may serve as a model for microenvironmental homing of IEL into the epithelium.

mucosa; homing; lymphocyte recirculation


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