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1 Division of Gastroenterology
and Nutrition,
The regulatory elements that control basal and
activated transcriptional expression of the polymeric IgA receptor gene
(pIgR) have not been defined. In
this study, we performed functional analysis of the murine
pIgR 5'-upstream region.
Transient transfection studies identified the gene's minimal promoter
to reside within 110 nucleotides upstream from the start of
transcription. Substitution mutations of this region identified both a
putative activator (
78 to 70) and a repressor (66
to 52) element. DNase I footprint analysis confirmed an area of
protection that spans from nucleotides 85 to 62. Mobility
shift assays of the putative region confirmed binding of upstream
stimulatory factor 1 (USF1) to an E box element at positions 75
and 70, representing the putative enhancer. Overexpression
studies using various forms of USF suggest that both USF1 and USF2
enhance activity of the pIgR minimal
promoter. We report the identification and characterization of the
murine pIgR minimal promoter, as well
as the critical role of USF in enhancing its basal level of
transcription in Caco-2 cells.
mucosa; transcription; immunoglobulin; upstream stimulatory factor; secretory component
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