AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 275: G854-G861, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stadnicki, A.
Right arrow Articles by Colman, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stadnicki, A.
Right arrow Articles by Colman, R. W.
Vol. 275, Issue 4, G854-G861, October 1998

Localization and secretion of tissue kallikrein in peptidoglycan-induced enterocolitis in Lewis rats

Antoni Stadnicki1, Julie Chao2, Iwona Stadnicka3, Eric Van Tol4, Kuei-Fu Lin2, Fengling Li4, R. Balfour Sartor4, and Robert W. Colman1

1 Sol Sherry Thrombosis Research Center and 3 Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140; 2 Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425; and 4 Department of Medicine, Center for Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, North Carolina 27599

The plasma kallikrein-kinin system is a mediator of intestinal inflammation induced by peptidoglycan-polysaccharide from group A streptococci (PG-APS) in rats. In this study we investigated the participation of intestinal tissue kallikrein (ITK). Lewis rats were injected intramurally with PG-APS. ITK was visualized by immunohistochemical staining. Cecal ITK concentration was measured by radioimmunoassay, and gene expression was evaluated by RNase protection assay. Kallikrein-binding protein (KBP) was evaluated in plasma by ELISA. Tissue kallikrein was identified in cecal goblet cells in both control and PG-APS-injected rats and in macrophages forming granulomas in inflamed tissues. Cecal ITK was significantly lower in acute and chronic phases of inflammation and in supernatant from in vitro cultures of inflamed cecum. ITK mRNA levels were not significantly different. Plasma KBP levels were significantly reduced in inflamed rats. The presence of tissue kallikrein in macrophages suggests participation in experimental colitis. The decrease of ITK in the inflamed intestine associated with unchanged mRNA levels suggests ITK release during intestinal inflammation.

inflammatory bowel diseases; macrophages; goblet cells; kallikrein-binding protein


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Stadnicki, E. Pastucha, G. Nowaczyk, U. Mazurek, D. Plewka, G. Machnik, T. Wilczok, and R. W. Colman
Immunolocalization and expression of kinin B1R and B2R receptors in human inflammatory bowel disease
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G361 - G366.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
D. M. Mutch, R. Simmering, D. Donnicola, G. Fotopoulos, J. A. Holzwarth, G. Williamson, and I. Corthesy-Theulaz
Impact of commensal microbiota on murine gastrointestinal tract gene ontologies
Physiol Genomics, September 16, 2004; 19(1): 22 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. T. Lauredo, R. M. Forteza, Y. Botvinnikova, and W. M. Abraham
Leukocytic cell sources of airway tissue kallikrein
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L734 - L740.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online