AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 275: G1018-G1027, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hillsley, K.
Right arrow Articles by Mawe, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hillsley, K.
Right arrow Articles by Mawe, G. M.
Vol. 275, Issue 5, G1018-G1027, November 1998

5-HT is present in nerves of guinea pig sphincter of Oddi and depolarizes sphincter of Oddi neurons

Kirk Hillsley and Gary M. Mawe

Department of Anatomy and Neurobiology, University of Vermont, Burlington, Vermont, 05405

This study involved immunohistochemistry and intracellular electrophysiology to investigate serotonergic neurotransmission in the sphincter of Oddi (SO). 5-Hydroxytryptamine (HT)-positive neurons (14 cells/preparation) and nerve fibers were observed in the ganglionated plexus. Serotonergic nerve fibers, which persisted under 2- to 6-day organ culture, were densely distributed, with varicose endings encircling some SO neurons. When 5-HT was applied to SO neurons, it elicited three different responses: 1) a fast depolarization to 5-HT in 31 of 62 cells was mimicked by 2-methyl-5-HT and blocked by LY-278584 (1 µM); 2) a prolonged depolarization to 5-HT in 21 of 62 cells evoked an increase in input resistance and was attenuated by the 5-HT1P antagonist renzapride (1 µM) but not by the 5-HT4 antagonist SDZ-205557 (0.1-10 µM); and 3) an indirect depolarization blocked by TTX or atropine was observed in 32 of 62 cells. 5-HT superfusion elicited a dose-dependent monophasic depolarization (EC50 = 2 µM, n=14). In conclusion, 5-HT is present in nerves of the SO and elicits both 5-HT3 and 5-HT1P receptor-mediated depolarizations, supporting the concept that 5-HT plays a role in SO regulation.

enteric nervous system; myenteric plexus; autonomic nervous system; biliary tract; serotonin


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. P. Manning and G. M. Mawe
Tachykinins mediate slow excitatory postsynaptic transmission in guinea pig sphincter of Oddi ganglia
Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G357 - G364.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. Vanden Berghe, J. Tack, A. Andrioli, L. Missiaen, and J. Janssens
Receptor-induced Ca2+ signaling in cultured myenteric neurons
Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G905 - G914.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. M. Mawe and A. L. Kennedy
Duodenal neurons provide nicotinic fast synaptic input to sphincter of Oddi neurons in guinea pig
Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G226 - G234.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online