| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Intestinal Disease Research
Program, Faculty of Health Sciences, McMaster University, Hamilton,
Ontario, Canada L8N 3Z5;
2 Institute of Neurobiology,
Evidence suggests that stress may be a contributing factor in intestinal inflammatory disease; however, the involved mechanisms have not been elucidated. We previously reported that acute stress alters epithelial physiology of rat intestine. In this study, we documented stress-induced macromolecular transport across intestinal epithelium. After exposure of Wistar-Kyoto rats to acute restraint stress, transport of a model protein, horseradish peroxidase (HRP), was assessed in isolated segments of jejunum. The flux of intact HRP was significantly enhanced across intestine from stressed rats compared with controls. Electron microscopy revealed HRP-containing endosomes within enterocytes, goblet cells, and Paneth cells of stressed rats. The number and area of HRP endosomes within enterocytes were found to be significantly increased by stress. HRP was also visualized in paracellular spaces between adjacent epithelial cells only in intestine from stressed rats. Atropine treatment of rats prevented the stress-induced abnormalities of protein transport. Our results suggest that stress, via a mechanism that involves release of acetylcholine, causes epithelial dysfunction that includes enhanced uptake of macromolecular protein antigens. We speculate that immune reactions to such foreign proteins may initiate or exacerbate inflammation.
enteric nerves; epithelium; permeability; endocytosis; tight junctions
This article has been cited by other articles:
|
|
 |
|
  E. Mazzon and S. Cuzzocrea Role of TNF-{alpha} in ileum tight junction alteration in mouse model of restraint stress Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1268 - G1280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Piche, M C Saint-Paul, R Dainese, E Marine-Barjoan, A Iannelli, M L Montoya, J F Peyron, D Czerucka, F Cherikh, J Filippi, et al. Mast cells and cellularity of the colonic mucosa correlated with fatigue and depression in irritable bowel syndrome Gut, April 1, 2008; 57(4): 468 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G Gareau, J. Jury, G. MacQueen, P. M Sherman, and M. H Perdue Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation Gut, November 1, 2007; 56(11): 1522 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Gareau, J. Jury, and M. H. Perdue Neonatal maternal separation of rat pups results in abnormal cholinergic regulation of epithelial permeability Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G198 - G203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Boudry, C. I. Cheeseman, and M. H. Perdue Psychological stress impairs Na+-dependent glucose absorption and increases GLUT2 expression in the rat jejunal brush-border membrane Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R862 - R867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Cameron, P.-C. Yang, and M. H. Perdue Glucagon-like peptide-2-enhanced barrier function reduces pathophysiology in a model of food allergy Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G905 - G912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Soderholm, D. A. Yates, M. G. Gareau, P.-C. Yang, G. MacQueen, and M. H. Perdue Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1257 - G1263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Mazzon, G C Sturniolo, D Puzzolo, N Frisina, and W Fries Effect of stress on the paracellular barrier in the rat ileum Gut, October 1, 2002; 51(4): 507 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S M COLLINS, T PICHE, and P RAMPAL The putative role of inflammation in the irritable bowel syndrome Gut, December 1, 2001; 49(6): 743 - 745. [Full Text] [PDF]
|
|
|
|
|
|
O. Nylander, A. Hallgren, and M. Sababi COX inhibition excites enteric nerves that affect motility, alkaline secretion, and permeability in rat duodenum Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1169 - G1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Yates, J. Santos, J. D. Soderholm, and M. H. Perdue Adaptation of stress-induced mucosal pathophysiology in rat colon involves opioid pathways Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G124 - G128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Santos, P-C Yang, J D Soderholm, M Benjamin, and M H Perdue Role of mast cells in chronic stress induced colonic epithelial barrier dysfunction in the rat Gut, May 1, 2001; 48(5): 630 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. M. Spreeuwenberg, J. M. A. J. Verdonk, H. R. Gaskins, and M. W. A. Verstegen Small Intestine Epithelial Barrier Function Is Compromised in Pigs with Low Feed Intake at Weaning J. Nutr., May 1, 2001; 131(5): 1520 - 1527. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Collins Stress and the Gastrointestinal Tract: IV. Modulation of intestinal inflammation by stress: basic mechanisms and clinical relevance Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G315 - G318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Soderholm and M. H. Perdue Stress and the Gastrointestinal Tract: II. Stress and intestinal barrier function Am J Physiol Gastrointest Liver Physiol, January 1, 2001; 280(1): G7 - G13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. MEDDINGS Barrier Dysfunction and Crohn's Disease Ann. N.Y. Acad. Sci., December 1, 2000; 915(1): 333 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E A MAYER The neurobiology of stress and gastrointestinal disease Gut, December 1, 2000; 47(6): 861 - 869. [Full Text] [PDF]
|
|
|
|
|
|
J Santos and M H Perdue Stress and neuroimmune regulation of gut mucosal function Gut, December 1, 2000; 47(90004): iv49 - 51. [Full Text] [PDF]
|
|
|
|
|
|
J. Karczewski and J. Groot Molecular Physiology and Pathophysiology of Tight Junctions III. Tight junction regulation by intracellular messengers: differences in response within and between epithelia Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G660 - G665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Santos, M. Benjamin, P.-C. Yang, T. Prior, and M. H. Perdue Chronic stress impairs rat growth and jejunal epithelial barrier function: role of mast cells Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G847 - G854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Santos, P. R. Saunders, N. P. M. Hanssen, P.-C. Yang, D. Yates, J. A. Groot, and M. H. Perdue Corticotropin-releasing hormone mimics stress-induced colonic epithelial pathophysiology in the rat Am J Physiol Gastrointest Liver Physiol, August 1, 1999; 277(2): G391 - G399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Perdue III. The mucosal antigen barrier: cross talk with mucosal cytokines Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G1 - G5. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
