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1 Institut National de la
Santé et de la Recherche Médicale U-45,
The effect of potential mediators of mucus
secretion was investigated in the isolated vascularly perfused rat
colon by using a sandwich enzyme-linked immunosorbent assay for rat
colonic mucin and by histochemical analysis. Bethanechol (100-200
µM), bombesin (100 nM), and vasoactive intestinal peptide (VIP, 100 nM) provoked a dramatic mucin discharge (maximal response at 900, 900, and 600% of control loops, respectively). VIP-stimulated mucin
secretion was abolished by tetrodotoxin, whereas atropine was without
effect. In contrast, both tetrodotoxin and atropine significantly
decreased mucin release induced by bombesin. Isoproterenol or
calcitonin gene-related peptide was without effect. Serotonin (1-5
µM) and peptide YY (10 nM) evoked mucin discharge, whereas
glucagon-like peptide-1 did not release mucin. Finally, bromolasalocid
(20 µM), interleukin-1
(0.25 nM), sodium nitroprusside (1 mM), and
dimethyl-PGE2 (2.5 µM) induced
mucus discharge. The results demonstrated a good correlation between
the immunological method and histological analysis. In conclusion,
these findings suggest a role for the enteric nervous system, the
enteroendocrine cells, and resident immune cells in mediation of
colonic mucus release.
colonic mucin release; enzyme-linked immunosorbent assay; cavitation; isolated colon
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