| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B activation is associated with hormone-induced
pancreatitis
Departments of 1 Medicine and 2 Surgery, Veterans Affairs Medical Center, West Los Angeles and University of California, Los Angeles, California 90073
Inflammation and cell death are critical to
pathogenesis of acute pancreatitis. Here we show that transcription
factor nuclear factor-
B (NF-B), which regulates these processes,
is activated and plays a role in rat cerulein pancreatitis. NF-B was
strongly activated in the pancreas within 30 min of cerulein infusion; a second phase of NF-B activation was prominent at 3-6 h. This biphasic kinetics could result from observed transient degradation of
the inhibitory protein IB
and slower but sustained degradation of
IB
. The hormone also caused NF-B translocation and IB
degradation in vitro in dispersed pancreatic acini. Both p65/p50 and
p50/p50, but not c-Rel, NF-B complexes were manifest in pancreatitis
and in isolated acini. Coinfusion of CCK JMV-180, which abolishes pancreatitis, prevented cerulein-induced NF-B activation. The second
but not early phase of NF-B activation was inhibited by a
neutralizing tumor necrosis factor- antibody. Antioxidant
N-acetylcysteine (NAC) blocked NF-B
activation and significantly improved parameters of pancreatitis. In
particular, NAC inhibited intrapancreatic trypsin activation and mRNA
expression of cytokines interleukin-6 and KC, which were dramatically
induced by cerulein. The results suggest that NF-B activation is an
important early event that may contribute to inflammatory and cell
death responses in acute pancreatitis.
pancreatic acinar cell; interleukin-6; KC; IB
This article has been cited by other articles:
|
|
 |
|
  Z Rakonczay Jr, P Hegyi, T Takacs, J McCarroll, and A K Saluja The role of NF-{kappa}B activation in the pathogenesis of acute pancreatitis Gut, February 1, 2008; 57(2): 259 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gukovsky, A. Lugea, M. Shahsahebi, J. H. Cheng, P. P. Hong, Y. J. Jung, Q.-g. Deng, B. A. French, W. Lungo, S. W. French, et al. A rat model reproducing key pathological responses of alcoholic chronic pancreatitis Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G68 - G79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, J. R. Reeve Jr., and G. M. Green Supramaximal CCK-58 does not induce pancreatitis in the rat: role of pancreatic water secretion Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G964 - G974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Satoh, A. S. Gukovskaya, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol Ethanol sensitizes NF-{kappa}B activation in pancreatic acinar cells through effects on protein kinase C-{epsilon} Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G432 - G438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Tietz, A. Malo, J. Diebold, A. Kotlyarov, A. Herbst, F. T. Kolligs, B. Brandt-Nedelev, W. Halangk, M. Gaestel, B. Goke, et al. Gene deletion of MK2 inhibits TNF-{alpha} and IL-6 and protects against cerulein-induced pancreatitis Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1298 - G1306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohashi, A. Nishio, H. Nakamura, M. Kido, S. Ueno, N. Uza, S. Inoue, H. Kitamura, K. Kiriya, M. Asada, et al. Protective roles of redox-active protein thioredoxin-1 for severe acute pancreatitis Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G772 - G781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Perides, A. Sharma, A. Gopal, X. Tao, K. Dwyer, B. Ligon, and M. L. Steer Secretin differentially sensitizes rat pancreatic acini to the effects of supramaximal stimulation with caerulein Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G713 - G721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Satoh, A. S. Gukovskaya, J. M. Nieto, J. H. Cheng, I. Gukovsky, J. R. Reeve Jr, T. Shimosegawa, and S. J. Pandol PKC-{delta} and -{epsilon} regulate NF-{kappa}B activation induced by cholecystokinin and TNF-{alpha} in pancreatic acinar cells Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G582 - G591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bhatia Apoptosis versus necrosis in acute pancreatitis Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G189 - G196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Gukovskaya, S. Hosseini, A. Satoh, J. H. Cheng, K. J. Nam, I. Gukovsky, and S. J. Pandol Ethanol differentially regulates NF-{kappa}B activation in pancreatic acinar cells through calcium and protein kinase C pathways Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G204 - G213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ji, X.-q. Chen, D. E. Misek, R. Kuick, S. Hanash, S. Ernst, R. Najarian, and C. D. Logsdon Pancreatic gene expression during the initiation of acute pancreatitis: identification of EGR-1 as a key regulator Physiol Genomics, June 24, 2003; 14(1): 59 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gukovsky, C. N. Reyes, E. C. Vaquero, A. S. Gukovskaya, and S. J. Pandol Curcumin ameliorates ethanol and nonethanol experimental pancreatitis Am J Physiol Gastrointest Liver Physiol, January 1, 2003; 284(1): G85 - G95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Han, H.-M. Ko, J.-H. Choi, K. H. Seo, H.-S. Lee, E.-K. Choi, I.-W. Choi, H.-K. Lee, and S.-Y. Im Molecular Mechanisms for Lipopolysaccharide-induced Biphasic Activation of Nuclear Factor-kappa B (NF-kappa B) J. Biol. Chem., November 15, 2002; 277(47): 44715 - 44721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Song, L. Bhagat, V. P. Singh, G. G. D. Van Acker, M. L. Steer, and A. K. Saluja Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1166 - G1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Algul, Y. Tando, M. Beil, C. K. Weber, C. Von Weyhern, G. Schneider, G. Adler, and R. M. Schmid Different modes of NF-kappa B/Rel activation in pancreatic lobules Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G270 - G281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Pausawasdi, S. Ramamoorthy, L. J. Crofford, F. K. Askari, and A. Todisco Regulation and function of COX-2 gene expression in isolated gastric parietal cells Am J Physiol Gastrointest Liver Physiol, June 1, 2002; 282(6): G1069 - G1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bhatia, M. Brady, Y. K. Kang, E. Costello, D. J. Newton, S. E. Christmas, J. P. Neoptolemos, and J. Slavin MCP-1 but not CINC synthesis is increased in rat pancreatic acini in response to cerulein hyperstimulation Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G77 - G85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hiraoka, Y. Miyazaki, S. Kitamura, M. Toyota, T. Kiyohara, Y. Shinomura, N. Mukaida, and Y. Matsuzawa Gastrin induces CXC chemokine expression in gastric epithelial cells through activation of NF-{kappa}B Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G735 - G742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gomez, H.-M. Lee, Q. He, E. W. Englander, T. Uchida, and G. H. Greeley Jr. Acute Pancreatitis Signals Activation of Apoptosis-Associated and Survival Genes inMice Experimental Biology and Medicine, July 1, 2001; 226(7): 692 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Frossard, C. M. Pastor, and A. Hadengue Effect of hyperthermia on NF-{kappa}B binding activity in cerulein-induced acute pancreatitis Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1157 - G1162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vaquero, I. Gukovsky, V. Zaninovic, A. S. Gukovskaya, and S. J. Pandol Localized pancreatic NF-{kappa}B activation and inflammatory response in taurocholate-induced pancreatitis Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1197 - G1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. PASTOR and J.-L. FROSSARD Are genetically modified mice useful for the understanding of acute pancreatitis? FASEB J, April 1, 2001; 15(6): 893 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Han, B. Ji, and C. D. Logsdon CCK independently activates intracellular trypsinogen and NF-{kappa}B in rat pancreatic acinar cells Am J Physiol Cell Physiol, March 1, 2001; 280(3): C465 - C472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Blinman, I. Gukovsky, M. Mouria, V. Zaninovic, E. Livingston, S. J. Pandol, and A. S. Gukovskaya Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1993 - C2003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Zaninovic, A. S. Gukovskaya, I. Gukovsky, M. Mouria, and S. J. Pandol Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G666 - G676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Han and C. D. Logsdon CCK stimulates mob-1 expression and NF-kappa B activation via protein kinase C and intracellular Ca2+ Am J Physiol Cell Physiol, February 1, 2000; 278(2): C344 - C351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tando, H. Algul, M. Wagner, H. Weidenbach, G. Adler, and R. M. Schmid Caerulein-induced NF-kappa B/Rel activation requires both Ca2+ and protein kinase C as messengers Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G678 - G686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Han and C. D. Logsdon Cholecystokinin induction of mob-1 chemokine expression in pancreatic acinar cells requires NF-kappa B activation Am J Physiol Cell Physiol, July 1, 1999; 277(1): C74 - C82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Hietaranta, V. P. Singh, L. Bhagat, G. J. D. van Acker, A. M. Song, A. Mykoniatis, M. L. Steer, and A. K. Saluja Water Immersion Stress Prevents Caerulein-induced Pancreatic Acinar Cell NF-kappa B Activation by Attenuating Caerulein-induced Intracellular Ca2+ Changes J. Biol. Chem., May 25, 2001; 276(22): 18742 - 18747. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
