AJP - GI Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 275: G1463-G1471, 1998;
0193-1857/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tham, D. M.
Right arrow Articles by Cohen, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tham, D. M.
Right arrow Articles by Cohen, H. J.
Vol. 275, Issue 6, G1463-G1471, December 1998

Expression of extracellular glutathione peroxidase in human and mouse gastrointestinal tract

Doris M. Tham, John C. Whitin, Kenneth K. Kim, Shirley X. Zhu, and Harvey J. Cohen

Department of Pediatrics, Stanford University School of Medicine, Palo Alto, California 94305

Extracellular glutathione peroxidase (EGPx) is a glycosylated selenoprotein capable of reducing hydrogen peroxide, organic hydroperoxides, free fatty acid hydroperoxides, and phosphatidylcholine hydroperoxides. We found that human large intestinal explant cultures synthesize EGPx and cellular glutathione peroxidase (CGPx) and secrete EGPx. The level of EGPx mRNA expression relative to alpha -tubulin was similar throughout the mouse gastrointestinal tract. EGPx mRNA transcripts have been localized to mature absorptive epithelial cells in human and mouse large intestine. Western blot analysis of mouse intestinal protein has demonstrated the presence of EGPx protein in the small intestine, cecum, and large intestine, with the highest protein levels found in the cecum. Immunohistochemistry studies of human large intestine and mouse small and large intestine sections demonstrated the presence of EGPx protein within mature absorptive epithelial cells. In human large intestine and mouse small intestine, EGPx protein is also present in the extracellular milieu. These results suggest a role for EGPx in protection of the intestinal tract from peroxidative damage and/or in intercellular metabolism of peroxides.

antioxidant; epithelial cells; oxidative damage; peroxides; selenium


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. A. A. Comhair and S. C. Erzurum
Antioxidant responses to oxidant-mediated lung diseases
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L246 - L255.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. S. Esworthy, R. Aranda, M. G. Martin, J. H. Doroshow, S. W. Binder, and F.-F. Chu
Mice with combined disruption of Gpx1 and Gpx2 genes have colitis
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G848 - G855.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. A. A. COMHAIR, P. R. BHATHENA, C. FARVER, F. B. J. M. THUNNISSEN, and S. C. ERZURUM
Extracellular glutathione peroxidase induction in asthmatic lungs: evidence for redox regulation of expression in human airway epithelial cells
FASEB J, January 1, 2001; 15(1): 70 - 78.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online