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Am J Physiol Gastrointest Liver Physiol 276: G107-G114, 1999;
0193-1857/99 $5.00
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Vol. 276, Issue 1, G107-G114, January 1999

Effect of sorbin on electrolyte transport in rat and human intestine

Bruno Eto1, Michel Boisset1, Bertrand Griesmar2, and Jehan-François Desjeux1,2

1 Conservatoire National des Arts et Métiers, Laboratoire de Biologie, 75141 Paris 03; and 2 Unité de Recherche sur les Fonctions Intestinales, le Métabolisme et la Nutrition, Institut National de la Santé et de la Recherche Médicale, Unité 290, Hôpital Saint-Lazare, 75010 Paris, France

Stimulating water absorption in the colon represents an important target to reduce stool output in secretory diarrhea. Recently, a 153-amino-acid peptide was isolated from porcine upper small intestine and purified, taking into account the increase of water absorption in guinea pig gallbladder. Accordingly, this peptide was named sorbin. The aim of the present study was to determine if the COOH-terminal heptapeptide of sorbin (C7-sorbin) participates in the regulation of electrolyte transport in the colon. Different regions (from duodenum to colon) of stripped intestinal mucosa from rats or humans were mounted in Ussing chambers to measure the changes in short-circuit current (Delta Isc) and net 22Na and 36Cl fluxes (JNanet and JClnet) after serosal exposure of 10-7 to 10-3 M C7-sorbin. In fasted rat intestine, C7-sorbin (10-4 M) induced an immediate reduction in Isc in the distal ileum and proximal and distal colon but not in the duodenum and jejunum. In the colon, Isc reduction and JNanet and JClnet stimulation were dose dependent (EC50 = 2 × 10-5 M). At 10-3 M, maximal effect was observed (Delta Isc = -1.14 ± 0.05, Delta JNanet = +4.97 ± 1.38, and Delta JClnet = +9.25 ± 1.44 µeq · h-1 · cm-2). C7-sorbin (10-3 M) inhibited the increase in Isc induced by a series of 10 secretory agents such as secretin, vasoactive intestinal peptide, PGE2, and serotonin. In HT-29-Cl19A cells, C7-sorbin induced an increase in Isc, with a maximal effect at 10-3 M (Delta Isc = 0.29 ± 0.10 µeq · h-1 · cm-2). In human intestine, a dose-dependent decrease in Isc was observed in right and sigmoid colons in basal and stimulated conditions (EC50 congruent  10-5 M; at 10-4 M, Delta Isc = -2.66 ± 0.17 µeq · h-1 · cm-2) but not in the jejunum. The results indicate that C7-sorbin stimulated NaCl neutral absorption and inhibited electrogenic Cl- in rat and human intestinal epithelia. In addition, the antisecretory effect was essentially observed in the distal part of both rat and human intestine and the magnitude of the proabsorptive effect was directly related to the magnitude of the previously induced secretion.

sodium chloride absorption; ion transport; colon


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F A. El Fadil-Nicol, F Berger, M Descroix-Vagne, and D Pansu
Presence of sorbin in human digestive tract and endocrine digestive tumours
Gut, February 1, 2000; 46(2): 182 - 190.
[Abstract] [Full Text] [PDF]


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