AJP - GI Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 276: G463-G469, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chung, B. M.
Right arrow Articles by Gall, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chung, B. M.
Right arrow Articles by Gall, D. G.
Vol. 276, Issue 2, G463-G469, February 1999

Role of actin in EGF-induced alterations in enterocyte SGLT1 expression

Brian M. Chung, Jason K. Wong, James A. Hardin, and D. Grant Gall

Gastrointestinal Research Group, Health Sciences Centre, University of Calgary, Calgary, Alberta, Canada T2N 4N1

Na+-glucose cotransporter (SGLT1) expression and the role of actin in epidermal growth factor (EGF)-induced alterations in glucose transport and brush-border surface area were examined in New Zealand White rabbit jejunal loops. In separate experiments, EGF or EGF concurrent with cytochalasin D, an inhibitor of actin polymerization, was administered to the experimental loop and compared with its vehicle control. SGLT1 expression was measured by Western blot in brush-border membrane vesicles (BBMV) after 5-min and 1-h exposure. Glucose kinetics were determined by a rapid filtration technique, and brush-border surface area was examined by electron microscopy after 1-h exposure. The effect of cytochalasin D alone on BBMV glucose kinetics and brush-border surface area was also assessed. EGF resulted in a significant increase in BBMV SGLT1 expression (P < 0.05), glucose maximal uptake (Vmax; P < 0.001), and absorptive brush-border surface area (P < 0.001). These effects were abolished with concurrent cytochalasin D treatment. Cytochalasin D alone had no effect on glucose transport or brush-border surface area. The findings suggest that EGF acutely upregulates jejunal brush-border surface area and the Vmax for jejunal glucose uptake via the recruitment and insertion of SGLT1 from an internal pool into the brush border by a mechanism that is dependent on actin polymerization.

epidermal growth factor; sodium-glucose cotransporter; glucose transport


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. A. Kles and K. A. Tappenden
Hypoxia differentially regulates nutrient transport in rat jejunum regardless of luminal nutrient present
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1336 - G1342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H.-L. Xu, D. L. Feinstein, R. A. Santizo, H. M. Koenig, and D. A. Pelligrino
Agonist-specific differences in mechanisms mediating eNOS-dependent pial arteriolar dilation in rats
Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H237 - H243.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
J Hardin, K Kroeker, B Chung, and D G Gall
Effect of proinflammatory interleukins on jejunal nutrient transport
Gut, August 1, 2000; 47(2): 184 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. G.-E. Scott, M. R. Logan, G. M. Klammer, D. A. Teoh, and A. G. Buret
Jejunal Brush Border Microvillous Alterations in Giardia muris-Infected Mice: Role of T Lymphocytes and Interleukin-6
Infect. Immun., June 1, 2000; 68(6): 3412 - 3418.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online