| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Nutrition and Foodservice Systems, University of North Carolina at Greensboro, Greensboro, North Carolina 27402; and 2 Mineral Bioavailability Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111
Calcium transport in the apical-to-basolateral
(A-to-B) or B-to-A direction was examined in cells treated with 10 nM
1,25-dihydroxyvitamin D3
[1,25(OH)2D3,
calcitriol] for up to 72 h. Net A-to-B calcium transport was
positive at all time points and increased from 0.14 ± 0.06 to 0.50 ± 0.01 nmol · well
1 · min1
after 72 h of calcitriol treatment. Neither phenol red transport nor
transepithelial electrical resistance was altered by calcitriol treatment, suggesting that the increase in net A-to-B calcium transport
was not due to paracellular movement. Neither 25-hydroxyvitamin D3 nor 24,25-dihydroxyvitamin
D3 (100 nM, 48 h) alters basal or calcitriol-stimulated A-to-B calcium transport. Treatment with the
calmodulin antagonist trifluoperazine (50 µM) reduced
calcitriol-stimulated A-to-B Ca transport by 56%. The transcription
inhibitor actinomycin D inhibited calcitriol-regulated A-to-B calcium
transport as well as calbindin D9k
and 24-hydroxylase mRNA accumulation. These data demonstrate that
calcitriol-mediated A-to-B calcium transport in Caco-2 cells is a
specific, transcellular process that requires transcriptional events
normally mediated through the vitamin D receptor.
trifluoperazine; 24-hydroxylase; calbindin D9k
This article has been cited by other articles:
|
|
 |
|
  H. Fujita, K. Sugimoto, S. Inatomi, T. Maeda, M. Osanai, Y. Uchiyama, Y. Yamamoto, T. Wada, T. Kojima, H. Yokozaki, et al. Tight Junction Proteins Claudin-2 and -12 Are Critical for Vitamin D-dependent Ca2+ Absorption between Enterocytes Mol. Biol. Cell, May 1, 2008; 19(5): 1912 - 1921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels Calcium Absorption Across Epithelia Physiol Rev, January 1, 2005; 85(1): 373 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, A. Klopot, J.-N. Freund, L. N. Dowling, S. D. Krasinski, and J. C. Fleet Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1{alpha} Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G943 - G953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Song, S. Kato, and J. C. Fleet Vitamin D Receptor (VDR) Knockout Mice Reveal VDR-Independent Regulation of Intestinal Calcium Absorption and ECaC2 and Calbindin D9k mRNA J. Nutr., February 1, 2003; 133(2): 374 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Kip and E. E. Strehler Characterization of PMCA isoforms and their contribution to transcellular Ca2+ flux in MDCK cells Am J Physiol Renal Physiol, January 1, 2003; 284(1): F122 - F132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Fleet, F. Eksir, K. W. Hance, and R. J. Wood Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G618 - G625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Tryfonidou, J. J. Stevenhagen, G.J.C.M. van den Bemd, M. A. Oosterlaken-Dijksterhuis, H. F. DeLuca, J. A. Mol, W. E. van den Brom, J.P.T.M. van Leeuwen, and H.A.W. Hazewinkel Moderate Cholecalciferol Supplementation Depresses Intestinal Calcium Absorption in Growing Dogs J. Nutr., September 1, 2002; 132(9): 2644 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-R. Jan and C.-J. Tseng W-7 Induces [Ca2+]i Increases in Madin-Darby Canine Kidney (MDCK) Cells J. Pharmacol. Exp. Ther., January 1, 2000; 292(1): 358 - 365. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
