AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 276: G1105-G1116, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kato, K.
Right arrow Articles by Soll, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kato, K.
Right arrow Articles by Soll, A. H.
Vol. 276, Issue 5, G1105-G1116, May 1999

Effects of growth factors and trefoil peptides on migration and replication in primary oxyntic cultures

Kimitoshi Kato1, Monica C. Chen1, Minh Nguyen1, Frank S. Lehmann1, Daniel K. Podolsky2, and Andrew H. Soll1

1 CURE: Digestive Diseases Research Center, West Los Angeles Veterans Affairs Medical Center, University of California Los Angeles School of Medicine, Los Angeles, California 90073; and 2 Gastrointestinal Unit, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts 02114

Restitution, the lateral migration of cells over an intact basement membrane, maintains mucosal integrity. We studied the regulation of migration and proliferation of enzyme-dispersed canine oxyntic mucosa cells in primary culture. Confluent monolayers were wounded and cultured in serum-free medium, and cells migrating into the wound were counted. [3H]thymidine incorporation into DNA was studied using subconfluent cultures. Considerable migration occurred in untreated monolayers; however, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha , basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), two trefoil peptides, and interleukin (IL)-1beta further enhanced migration. The specific EGF receptor (EGFR) monoclonal antibody, MAb-528, inhibited both basal and TGF-alpha - or IL-1beta -stimulated migration, but not the response to trefoil peptide, bFGF, or IGF-I. Exogenous TGF-beta inhibited cell proliferation but did not alter migration. Immunoneutralization with anti-TGF-beta blocked the response to exogenous TGF-beta and produced a small enhancement of basal thymidine incorporation but did not attenuate basal or TGF-alpha -stimulated migration. In conclusion, endogenous EGFR ligands regulate proliferation and migration. TGF-beta inhibits mitogenesis; it did not upregulate migration in these cultures. Although bFGF, IGF-I, and IL-1beta enhance gastric epithelial migration, only IL-1beta acted in a TGF-alpha -dependent fashion.

gastric mucosa; thymidine incorporation; epidermal growth factor receptors; epidermal growth factor receptor antibody; peptic ulcer; cytokines


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. Gunzel, P. Florian, J. F. Richter, H. Troeger, J. D. Schulzke, M. Fromm, and A. H. Gitter
Restitution of single-cell defects in the mouse colon epithelium differs from that of cultured cells
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1496 - R1507.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Ogawa, S. Nomura, A. Varro, T. C. Wang, and J. R. Goldenring
Altered metaplastic response of waved-2 EGF receptor mutant mice to acute oxyntic atrophy
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G793 - G804.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Mareel and A. Leroy
Clinical, Cellular, and Molecular Aspects of Cancer Invasion
Physiol Rev, April 1, 2003; 83(2): 337 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. C. Chen, T. E. Solomon, R. Kui, and A. H. Soll
Apical EGF receptors regulate epithelial barrier to gastric acid: endogenous TGF-alpha is an essential facilitator
Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1098 - G1106.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. C. Chen, T. E. Solomon, E. P. Salazar, R. Kui, E. Rozengurt, and A. H. Soll
Secretin regulates paracellular permeability in canine gastric monolayers by a Src kinase-dependent pathway
Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G893 - G899.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. M. Kirton, T. Wang, and G. J. Dockray
Regulation of parietal cell migration by gastrin in the mouse
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G787 - G793.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
F. S. Lehmann, L. Terracciano, I. Carena, C. Baeriswyl, J. Drewe, L. Tornillo, G. De Libero, and C. Beglinger
In situ correlation of cytokine secretion and apoptosis in Helicobacter pylori-associated gastritis
Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G481 - G488.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. L. Gookin, J. M. Rhoads, and R. A. Argenzio
Inducible nitric oxide synthase mediates early epithelial repair of porcine ileum
Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G157 - G168.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
I. L. Szabo, R. Pai, M. K. Jones, G. R. Ehring, H. Kawanaka, and A. S. Tarnawski
Indomethacin Delays Gastric Restitution: Association with the Inhibition of Focal Adhesion Kinase and Tensin Phosphorylation and Reduced Actin Stress Fibers
Experimental Biology and Medicine, June 1, 2002; 227(6): 412 - 424.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. EMAMI, N. LE FLOCH, E. BRUYNEEL, L. THIM, F. MAY, B. WESTLEY, M.-C. RIO, M. MAREEL, and C. GESPACH
Induction of scattering and cellular invasion by trefoil peptides in src- and RhoA-transformed kidney and colonic epithelial cells
FASEB J, February 1, 2001; 15(2): 351 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. C. Chen, J. Goliger, N. Bunnett, and A. H. Soll
Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability
Am J Physiol Gastrointest Liver Physiol, February 1, 2001; 280(2): G264 - G272.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. R. Turner, L. Liu, S. E. G. Fligiel, R. Jaszewski, and A. P. N. Majumdar
Aging alters gastric mucosal responses to epidermal growth factor and transforming growth factor-alpha
Am J Physiol Gastrointest Liver Physiol, May 1, 2000; 278(5): G805 - G810.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online