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1 Department of Physiology,
This study was undertaken to
investigate the requirement of
2-integrins (CD11/CD18) for
extravasation of neutrophils in mice. After intraperitoneal
thioglycollate injection, an in vivo model of inflammation, leukocyte
extravasation into the peritoneal cavity was studied in CD18-deficient
and wild-type control mice. Before the induction of peritonitis, total
and differential leukocyte counts in the circulation were analyzed and
found to be 10-fold elevated in CD18-deficient animals compared with
wild-type animals. This was largely due to neutrophilia, with a 30-fold
increase in neutrophil counts. In CD18-deficient animals, extravasated white blood cells in the peritoneal cavity were diminished by 46%. The neutrophil number in the peritoneal fluid was
severely reduced to 13% compared with control animals. In contrast,
the number of emigrated monocytes was enhanced and lymphocyte
emigration was not altered in the absence of CD18. The emigration
efficiency of the neutrophils, calculated as ratio of the cell number
in the lavage fluid and the circulating blood, was reduced to 0.4% in
CD18-deficient mice compared with wild-type animals. Thus efficient neutrophil emigration into the peritoneal cavity strongly required CD11/CD18.
inflammation; cell adhesion; CD18 antigen; host defense
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