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Am J Physiol Gastrointest Liver Physiol 276: G1204-G1212, 1999;
0193-1857/99 $5.00
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Vol. 276, Issue 5, G1204-G1212, May 1999

Calcium waves in colonic myocytes produced by mechanical and receptor-mediated stimulation

S. H. Young, H. S. Ennes, J. A. McRoberts, V. V. Chaban, S. K. Dea, and E. A. Mayer

CURE: Digestive Diseases Research Center/Neuroenteric Disease Program, Departments of Medicine and Physiology, University of California at Los Angeles, Los Angeles, California 90024

The mechanisms underlying intracellular Ca2+ waves induced by either mechanical or receptor-mediated stimulation of myocytes isolated from the longitudinal muscle layer of the rabbit distal colon were compared using fura 2 and fluorescence videomicroscopy. Light focal mechanical deformation of the plasma membrane or focal application of substance P resulted in localized intracellular Ca2+ concentration ([Ca2+]i) transients that propagated throughout the cell. In both cases, the Ca2+ response consisted of a transient peak response followed by a delayed-phase response. Substance P-mediated [Ca2+]i responses involved generation of inositol 1,4,5-trisphosphate and release of Ca2+ from thapsigargin-sensitive stores, whereas mechanically induced responses were partially (29%) dependent on La3+-sensitive influx of extracellular Ca2+ and partially on release of intracellular Ca2+ from thapsigargin-insensitive stores gated by ryanodine receptors. The delayed-phase response in both cases was dependent on extracellular Ca2+. However, although the response to substance P was sensitive to La3+, that after mechanical stimulation was not. In the later case, the underlying mechanism may involve capacitative Ca2+ entry channels that are activated after mechanical stimulation but not by substance P.

smooth muscle; tissue culture; substance P; inositol 1,4,5-trisphosphate; ryanodine receptor; stretch-activated cation channels


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