AJP - GI Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 276: G1289-G1301, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LeSage, G. D.
Right arrow Articles by Alpini, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LeSage, G. D.
Right arrow Articles by Alpini, G.
Vol. 276, Issue 5, G1289-G1301, May 1999

Acute carbon tetrachloride feeding induces damage of large but not small cholangiocytes from BDL rat liver

Gene D. LeSage1, Shannon S. Glaser2, Luca Marucci3, Antonio Benedetti3, Jo Lynne Phinizy2, Rebecca Rodgers4, Alessandra Caligiuri1, Emanuela Papa1, Ziga Tretjak1, Anne-Marie Jezequel5, Leigh A. Holcomb6, and Gianfranco Alpini1,4,7

1 Department of Internal Medicine, 2 Division of Research and Education and 4 Medical Physiology and 6 Department of Psychiatry and Behavioral Science, Scott & White Hospital and The Texas A&M University System Health Science Center College of Medicine and 7 Central Texas Veterans Health Care System, Temple, Texas 76504; and 3 Department of Gastroenterology, and 5 Institute of Experimental Pathology, University of Ancona, Ancona, Italy 60020

Bile duct damage and/or loss is limited to a range of duct sizes in cholangiopathies. We tested the hypothesis that CCl4 damages only large ducts. CCl4 or mineral oil was given to bile duct-ligated (BDL) rats, and 1, 2, and 7 days later small and large cholangiocytes were purified and evaluated for apoptosis, proliferation, and secretion. In situ, we measured apoptosis by morphometric and TUNEL analysis and the number of small and large ducts by morphometry. Two days after CCl4 administration, we found an increased number of small ducts and reduced number of large ducts. In vitro apoptosis was observed only in large cholangiocytes, and this was accompanied by loss of proliferation and secretion in large cholangiocytes and loss of choleretic effect of secretin. Small cholangiocytes de novo express the secretin receptor gene and secretin-induced cAMP response. Consistent with damage of large ducts, we detected cytochrome P-4502E1 (which CCl4 converts to its radicals) only in large cholangiocytes. CCl4 induces selective apoptosis of large ducts associated with loss of large cholangiocyte proliferation and secretion.

intrahepatic biliary tree; morphometry; proliferation; secretin receptor; adenosine 3',5'-cyclic monophosphate; bile secretion


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Mancinelli, P. Onori, E. Gaudio, S. DeMorrow, A. Franchitto, H. Francis, S. Glaser, G. Carpino, J. Venter, D. Alvaro, et al.
Follicle-stimulating hormone increases cholangiocyte proliferation by an autocrine mechanism via cAMP-dependent phosphorylation of ERK1/2 and Elk-1
Am J Physiol Gastrointest Liver Physiol, July 1, 2009; 297(1): G11 - G26.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
R. Mancinelli, P. Onori, E. Gaudio, A. Franchitto, G. Carpino, Y. Ueno, D. Alvaro, L. P Annarale, S. DeMorrow, and H. Francis
Taurocholate Feeding to Bile Duct Ligated Rats Prevents Caffeic Acid-Induced Bile Duct Damage by Changes in Cholangiocyte VEGF Expression
Experimental Biology and Medicine, April 1, 2009; 234(4): 462 - 474.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Francis, S. Glaser, S. DeMorrow, E. Gaudio, Y. Ueno, J. Venter, D. Dostal, P. Onori, A. Franchitto, M. Marzioni, et al.
Small mouse cholangiocytes proliferate in response to H1 histamine receptor stimulation by activation of the IP3/CaMK I/CREB pathway
Am J Physiol Cell Physiol, August 1, 2008; 295(2): C499 - C513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Glaser, S. DeMorrow, H. Francis, Y. Ueno, E. Gaudio, S. Vaculin, J. Venter, A. Franchitto, P. Onori, B. Vaculin, et al.
Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms
Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G124 - G136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. DeMorrow, H. Francis, E. Gaudio, Y. Ueno, J. Venter, P. Onori, A. Franchitto, B. Vaculin, S. Vaculin, and G. Alpini
Anandamide inhibits cholangiocyte hyperplastic proliferation via activation of thioredoxin 1/redox factor 1 and AP-1 activation
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G506 - G519.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Francis, G. LeSage, S. DeMorrow, D. Alvaro, Y. Ueno, J. Venter, S. Glaser, M. G. Mancino, L. Marucci, A. Benedetti, et al.
The {alpha}2-adrenergic receptor agonist UK 14,304 inhibits secretin-stimulated ductal secretion by downregulation of the cAMP system in bile duct-ligated rats
Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1252 - C1262.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
Y. Ueno, H. Francis, S. Glaser, S. DeMorrow, J. Venter, A. Benedetti, G. Fava, M. Marzioni, and G. Alpini
Taurocholic Acid Feeding Prevents Tumor Necrosis Factor-{alpha}-Induced Damage of Cholangiocytes by a PI3K-Mediated Pathway
Experimental Biology and Medicine, July 1, 2007; 232(7): 942 - 949.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Fava, Y. Ueno, S. Glaser, H. Francis, S. DeMorrow, L. Marucci, M. Marzioni, A. Benedetti, J. Venter, B. Vaculin, et al.
Thyroid hormone inhibits biliary growth in bile duct-ligated rats by PLC/IP3/Ca2+-dependent downregulation of SRC/ERK1/2
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1467 - C1475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. Fukushima, Y. Ueno, J. Inoue, N. Kanno, and T. Shimosegawa
Filopodia formation via a specific Eph family member and PI3K in immortalized cholangiocytes
Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G812 - G819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Gaudio, B. Barbaro, D. Alvaro, S. Glaser, H. Francis, A. Franchitto, P. Onori, Y. Ueno, M. Marzioni, G. Fava, et al.
Administration of r-VEGF-A prevents hepatic artery ligation-induced bile duct damage in bile duct ligated rats
Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G307 - G317.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Glaser, D. Alvaro, H. Francis, Y. Ueno, L. Marucci, A. Benedetti, S. D. Morrow, M. Marzioni, M. G. Mancino, J. L. Phinizy, et al.
Adrenergic receptor agonists prevent bile duct injury induced by adrenergic denervation by increased cAMP levels and activation of Akt
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G813 - G826.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Alpini, Y. Ueno, L. Tadlock, S. S. Glaser, G. LeSage, H. Francis, S. Taffetani, M. Marzioni, D. Alvaro, and T. Patel
Increased susceptibility of cholangiocytes to tumor necrosis factor-{alpha} cytotoxicity after bile duct ligation
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C183 - C194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Marzioni, G. D. LeSage, S. Glaser, T. Patel, C. Marienfeld, Y. Ueno, H. Francis, D. Alvaro, L. Tadlock, A. Benedetti, et al.
Taurocholate prevents the loss of intrahepatic bile ducts due to vagotomy in bile duct-ligated rats
Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G837 - G852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Glaser, D. Alvaro, T. Roskams, J. L. Phinizy, G. Stoica, H. Francis, Y. Ueno, B. Barbaro, M. Marzioni, J. Mauldin, et al.
Dopaminergic inhibition of secretin-stimulated choleresis by increased PKC-gamma expression and decrease of PKA activity
Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G683 - G694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Marucci, G. Alpini, S. S. Glaser, D. Alvaro, A. Benedetti, H. Francis, J. L. Phinizy, M. Marzioni, J. Mauldin, J. Venter, et al.
Taurocholate feeding prevents CCl4-induced damage of large cholangiocytes through PI3-kinase-dependent mechanism
Am J Physiol Gastrointest Liver Physiol, February 1, 2003; 284(2): G290 - G301.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. Kanno, G. LeSage, S. Glaser, and G. Alpini
Regulation of cholangiocyte bicarbonate secretion
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G612 - G625.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. Lesage, S. Glaser, Y. Ueno, D. Alvaro, L. Baiocchi, N. Kanno, J. L. Phinizy, H. Francis, and G. Alpini
Regression of cholangiocyte proliferation after cessation of ANIT feeding is coupled with increased apoptosis
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G182 - G190.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Paku, J. Schnur, P. Nagy, and S. S. Thorgeirsson
Origin and Structural Evolution of the Early Proliferating Oval Cells in Rat Liver
Am. J. Pathol., April 1, 2001; 158(4): 1313 - 1323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online