AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 277: G183-G190, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kamimura, H.
Right arrow Articles by Takeuchi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamimura, H.
Right arrow Articles by Takeuchi, T.
Vol. 277, Issue 1, G183-G190, July 1999

Kex2 family endoprotease furin is expressed specifically in pit-region parietal cells of the rat gastric mucosa

Hitoshi Kamimura1,2, Yoshitaka Konda1, Hiromi Yokota1, Sei-Ichi Takenoshita2, Yukio Nagamachi2, Hiroyuki Kuwano2, and Toshiyuki Takeuchi1

1 Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, and 2 First Department of Surgery, Gunma University School of Medicine, Maebashi 371, Japan

The proprotein-processing endoprotease furin is localized in the gastric epithelial cells of the pit region in the rat gastric gland. The gastric pit is composed of several cell types, including gastric surface mucosal (GSM) cells and parietal cells. Furin converts many growth- or differentiation-related proproteins to their active forms. We examined identification of furin-positive cells by immunostaining of rat gastric mucosa and regulators of the furin expression by measuring the furin promoter activity by luciferase assay. Furin-positive cells were stained for H+-K+-ATPase, indicating that they are parietal cells. Furin-positive parietal cells were not stained for transforming growth factor-alpha (TGF-alpha ) but were surrounded by TGF-alpha -positive GSM cells. In contrast, parietal cells below the proliferative zone were positive for TGF-alpha but not for furin. Furin-positive parietal cells expressed a high level of epidermal growth factor receptor (EGFR). TGF-alpha stimulated the furin promoter activity highly in a mouse GSM cell line GSM06. Thus we suggest that the parietal cells of the pit region have furin-mediated functions that can be stimulated by EGFR signaling.

transforming growth factor-alpha ; gastric surface mucous cells; gastric pit


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Ogawa, S. Nomura, A. Varro, T. C. Wang, and J. R. Goldenring
Altered metaplastic response of waved-2 EGF receptor mutant mice to acute oxyntic atrophy
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G793 - G804.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Negishi, D. Lu, Y.-Q. Zhang, Y. Sawada, T. Sasaki, T. Kayo, J. Ando, T. Izumi, M. Kurabayashi, I. Kojima, et al.
Upregulatory Expression of Furin and Transforming Growth Factor-{beta} by Fluid Shear Stress in Vascular Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 785 - 790.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Nakajima, Y. Konda, Y. Izumi, M. Kanai, N. Hayashi, T. Chiba, and T. Takeuchi
Gastrin stimulates the growth of gastric pit cell precursors by inducing its own receptors
Am J Physiol Gastrointest Liver Physiol, February 1, 2002; 282(2): G359 - G366.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online