AJP - GI Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 277: G321-G332, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alper, S. L.
Right arrow Articles by Seidler, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alper, S. L.
Right arrow Articles by Seidler, U.
Vol. 277, Issue 2, G321-G332, August 1999

Expression of AE2 anion exchanger in mouse intestine

Seth L. Alper1,2,3, Heidi Rossmann4, Sabine Wilhelm1, Alan K. Stuart-Tilley1, Boris E. Shmukler1,2, and Ursula Seidler4

1 Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center; Departments of 2 Medicine and 3 Cell Biology, Harvard Medical School, Boston, Massachusetts 02215; and 4 Department of Medicine, Eberhard-Karls Universitat, 72076 Tuebingen, Germany

We have characterized expression of anion exchanger 2 (AE2) mRNA and protein in the mouse intestine. AE2 mRNA abundance was higher in colon than in more proximal segments. AE2a mRNA was more abundant than AE2b mRNA throughout the intestine, and AE2c mRNA was expressed at very low levels. This AE2 mRNA pattern contrasted with that in mouse stomach, in which AE2c > AE2b > AE2a. AE2 polypeptide abundance as detected by immunoblot qualitatively paralleled that of mRNA, whereas AE2 immunostaining exhibited a more continuous decrease in intensity from colon to duodenum. AE2 polypeptide was more abundant in colonic surface cells than in crypts, whereas ileal crypts and villi exhibited similar AE2 abundance. AE2 was also observed in mural and vascular smooth muscle. Localization of AE2 epitopes was restricted to the basolateral membranes of epithelial cells throughout the intestine with three exceptions. Under mild fixation conditions, anti-AE2 amino acids (aa) 109-122 detected nonpolarized immunostaining of ileal enterocytes and of Paneth cell granule membranes. An epitope detected by anti-AE2 aa 1224-1237 was also localized to subapical regions of Brunner's gland ducts of duodenum and upper jejunum. These localization studies will aid in the interpretation of anion exchanger function measured in epithelial sheets, isolated cells, and membrane vesicles.

enterocytes; Paneth cells; Brunner's glands; chloride-bicarbonate exchange; immunocytochemistry


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
P. E. Morgan, S. Pastorekova, A. K. Stuart-Tilley, S. L. Alper, and J. R. Casey
Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C738 - C748.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H. Zhang, N. Ameen, J. E. Melvin, and S. Vidyasagar
Acute inflammation alters bicarbonate transport in mouse ileum
J. Physiol., June 15, 2007; 581(3): 1221 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Pushkin and I. Kurtz
SLC4 base (HCO3-, CO32-) transporters: classification, function, structure, genetic diseases, and knockout models
Am J Physiol Renal Physiol, March 1, 2006; 290(3): F580 - F599.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. T. Worrell, A. Best, O. R. Crawford, J. Xu, M. Soleimani, and J. B. Matthews
Apical ammonium inhibition of cAMP-stimulated secretion in T84 cells is bicarbonate dependent
Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G768 - G778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. E. Shmukler, C. E. Kurschat, G. E. Ackermann, L. Jiang, Y. Zhou, B. Barut, A. K. Stuart-Tilley, J. Zhao, L. I. Zon, I. A. Drummond, et al.
Zebrafish slc4a2/ae2 anion exchanger: cDNA cloning, mapping, functional characterization, and localization
Am J Physiol Renal Physiol, October 1, 2005; 289(4): F835 - F849.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Spiegel, M. Phillipper, H. Rossmann, B. Riederer, M. Gregor, and U. Seidler
Independence of apical Cl-/HCO3- exchange and anion conductance in duodenal HCO3- secretion
Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G887 - G897.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Ikuma, J. Geibel, H. J. Binder, and V. M. Rajendran
Characterization of Cl-HCO3 exchange in basolateral membrane of rat distal colon
Am J Physiol Cell Physiol, October 1, 2003; 285(4): C912 - C921.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. N. Chernova, A. K. Stewart, L. Jiang, D. J. Friedman, Y. Z. Kunes, and S. L. Alper
Structure-function relationships of AE2 regulation by Ca2+i-sensitive stimulators NH+4 and hypertonicity
Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1235 - C1246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. Bachmann, H. Rossmann, U. V. Berger, W. H. Colledge, R. Ratcliff, M. J. Evans, M. Gregor, and U. Seidler
cAMP-mediated regulation of murine intestinal/pancreatic Na+/HCO3- cotransporter subtype pNBC1
Am J Physiol Gastrointest Liver Physiol, January 1, 2003; 284(1): G37 - G45.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Turi, I. Jaspers, L. A. Dailey, M. C. Madden, L. E. Brighton, J. D. Carter, E. Nozik-Grayck, C. A. Piantadosi, and A. J. Ghio
Oxidative stress activates anion exchange protein 2 and AP-1 in airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L791 - L798.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ayabe, D. P. Satchell, P. Pesendorfer, H. Tanabe, C. L. Wilson, S. J. Hagen, and A. J. Ouellette
Activation of Paneth Cell alpha -Defensins in Mouse Small Intestine
J. Biol. Chem., February 8, 2002; 277(7): 5219 - 5228.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Greeley, H. Shumaker, Z. Wang, C. W. Schweinfest, and M. Soleimani
Downregulated in adenoma and putative anion transporter are regulated by CFTR in cultured pancreatic duct cells
Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1301 - G1308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Repishti, D. L. Hogan, V. Pratha, L. Davydova, M. Donowitz, C. M. Tse, and J. I. Isenberg
Human duodenal mucosal brush border Na+/H+ exchangers NHE2 and NHE3 alter net bicarbonate movement
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G159 - G163.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
K. Holappa, M. Suokas, P. Soininen, and S. Kellokumpu
Identification of the Full-length AE2 (AE2a) Isoform as the Golgi-associated Anion Exchanger in Fibroblasts
J. Histochem. Cytochem., February 1, 2001; 49(2): 259 - 270.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
V. M. Rajendran, J. Black, T. A. Ardito, P. Sangan, S. L. Alper, C. Schweinfest, M. Kashgarian, and H. J. Binder
Regulation of DRA and AE1 in rat colon by dietary Na depletion
Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G931 - G942.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. L. Clarke, M. C. Harline, L. R. Gawenis, N. M. Walker, J. T. Turner, and G. A. Weisman
Extracellular UTP stimulates electrogenic bicarbonate secretion across CFTR knockout gallbladder epithelium
Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G132 - G138.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Roussa, M. F. Romero, B. M. Schmitt, W. F. Boron, S. L. Alper, and F. Thevenod
Immunolocalization of anion exchanger AE2 and Na+-HCO-3 cotransporter in rat parotid and submandibular glands
Am J Physiol Gastrointest Liver Physiol, December 1, 1999; 277(6): G1288 - G1296.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online