AJP - GI Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 277: G478-G486, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eskandari, M. K.
Right arrow Articles by Bauer, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eskandari, M. K.
Right arrow Articles by Bauer, A. J.
Vol. 277, Issue 2, G478-G486, August 1999

LPS-induced muscularis macrophage nitric oxide suppresses rat jejunal circular muscle activity

Mark K. Eskandari, Jörg C. Kalff, Timothy R. Billiar, Kenneth K. W. Lee, and Anthony J. Bauer

Division of Gastroenterology, Departments of Surgery and Medicine, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania 15261

Cellular mechanisms of sepsis-induced ileus remain an enigma. The study aim was to determine the role of nitric oxide (NO) in mediating the suppression of rat jejunal circular smooth muscle activity during endotoxemia. Isolated muscularis inducible NO synthase (iNOS) mRNA was measured by RT-PCR, immunohistochemistry was employed to localize iNOS protein, and contractile activity was measured in an organ bath. The low basal expression of muscularis iNOS mRNA expression was increased in a time-dependent fashion after lipopolysaccharide (LPS), resulting in a 20-fold increase over controls 3 h after injection. Immunohistochemistry of muscularis whole mounts and dissociated muscularis cells for iNOS revealed staining only in the muscularis macrophages 12 h after LPS. LPS caused a 68% reduction in spontaneous muscle activity 12 h after injection, which improved by 53% after the in vitro application of the selective iNOS inhibitor L-N6-(1-iminoethyl)lysine. Similar results were obtained in C57BL/6 mice but not in iNOS knockout mice. These data demonstrate that macrophage iNOS plays an important role in mediating LPS-induced intestinal circular muscle suppression.

sepsis; inducible nitric oxide; small intestine; macrophage; smooth muscle; lipopolysaccharide


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Hori, H. Nobe, K. Horiguchi, and H. Ozaki
MCP-1 targeting inhibits muscularis macrophage recruitment and intestinal smooth muscle dysfunction in colonic inflammation
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C391 - C401.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ohama, M. Hori, E. Momotani, M. Elorza, W. T. Gerthoffer, and H. Ozaki
IL-1beta inhibits intestinal smooth muscle proliferation in an organ culture system: involvement of COX-2 and iNOS induction in muscularis resident macrophages
Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1315 - G1322.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Overhaus, B. A. Moore, J. E. Barbato, F. F. Behrendt, J. G. Doering, and A. J. Bauer
Biliverdin protects against polymicrobial sepsis by modulating inflammatory mediators
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G695 - G703.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Overhaus, S. Togel, M. A. Pezzone, and A. J. Bauer
Mechanisms of polymicrobial sepsis-induced ileus
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G685 - G694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Suzuki, K.-J. Won, K. Horiguchi, K. Kinoshita, M. Hori, S. Torihashi, E. Momotani, K. Itoh, K. Hirayama, S. M. Ward, et al.
Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G638 - G646.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
A Nakao, B A Moore, N Murase, F Liu, B S Zuckerbraun, F H Bach, A M K Choi, M A Nalesnik, L E Otterbein, and A J Bauer
Immunomodulatory effects of inhaled carbon monoxide on rat syngeneic small bowel graft motility
Gut, September 1, 2003; 52(9): 1278 - 1285.
[Abstract] [Full Text]

Home page
 GutHome page
R C Spiller
Role of nerves in enteric infection
Gut, December 1, 2002; 51(6): 759 - 762.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. M. Sanders, T. Ordog, and S. M. Ward
Physiology and Pathophysiology of the Interstitial Cells of Cajal: From Bench to Bedside: IV. Genetic and animal models of GI motility disorders caused by loss of interstitial cells of Cajal
Am J Physiol Gastrointest Liver Physiol, May 1, 2002; 282(5): G747 - G756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Hori, M. Kita, S. Torihashi, S. Miyamoto, K.-J. Won, K. Sato, H. Ozaki, and H. Karaki
Upregulation of iNOS by COX-2 in muscularis resident macrophage of rat intestine stimulated with LPS
Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G930 - G938.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Turler, N. T. Schwarz, E. Turler, J. C. Kalff, and A. J. Bauer
MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat
Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G145 - G155.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online