AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 277: G819-G828, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sato, Y.
Right arrow Articles by Suzuki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sato, Y.
Right arrow Articles by Suzuki, Y.
Vol. 277, Issue 4, G819-G828, October 1999

Role of the vasopressin V1 receptor in regulating the epithelial functions of the guinea pig distal colon

Yoshihiko Sato1, Hiroyuki Hanai2, Atsuhiro Nogaki1, Koki Hirasawa1, Eizo Kaneko1, Hisayoshi Hayashi3, and Yuichi Suzuki3

1 First Department of Medicine, 2 Department of Endoscopic and Photodynamic Medicine, Hamamatsu University School of Medicine, Hamamatsu 431-3192; and 3 Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan

Vasopressin has a wide spectrum of biological action. In this study, the role of vasopressin in regulating electrolyte transport in the colon was elucidated by measuring the short-circuit current (Isc) as well as the Na+, K+, and Cl- flux in a chamber-mounted mucosal sheet. The cytosolic Ca2+ concentration ([Ca2+]i) was also measured in fura 2-loaded cells by fluorescence imaging. Serosal vasopressin decreased Isc at 10-9 M and increased Isc at 10-7-10-6 M. The decrease in Isc was accompanied by two effects: one was a decrease in the amiloride-sensitive Na+ absorption, whereas the other was an increase in the bumetanide-sensitive K+ secretion. The increase in Isc was accompanied by an increase in the Cl- secretion that can be inhibited by serosal bumetanide or mucosal diphenylamine-2-carboxylate. Vasopressin caused an increase in [Ca2+]i in crypt cells. These responses of Isc and the [Ca2+]i increase in crypt cells were all more potently inhibited by the vasopressin V1 receptor antagonist than by the V2 receptor antagonist. These results suggest that vasopressin inhibits electrogenic Na+ absorption and stimulates electrogenic K+ and Cl- secretion. In all of these responses, the V1 receptor is involved, and the [Ca2+]i increase may play an important role.

sodium absorption; potassium secretion; chloride secretion; intracellular calcium; intestinal transport


This article has been cited by other articles:


Home page
 J. Physiol.Home page
E. Cristia, C. Amat, R. J. Naftalin, and M. Moreto
Role of vasopressin in rat distal colon function
J. Physiol., January 15, 2007; 578(2): 413 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. M. Blumenthal
Modulation of tyramine signaling by osmolality in an insect secretory epithelium
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1261 - C1267.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Hayashi, T. Suzuki, T. Yamamoto, and Y. Suzuki
Cholinergic inhibition of electrogenic sodium absorption in the guinea pig distal colon
Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G617 - G628.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Yamamoto and Y. Suzuki
Role of luminal ATP in regulating electrogenic Na+ absorption in guinea pig distal colon
Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G300 - G308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Chiu, S. S. Wu, C. Santiskulvong, P. Tangkijvanich, H. F. Yee Jr., and E. Rozengurt
Vasopressin-mediated mitogenic signaling in intestinal epithelial cells
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C434 - C450.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online