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Am J Physiol Gastrointest Liver Physiol 277: G819-G828, 1999;
0193-1857/99 $5.00
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Vol. 277, Issue 4, G819-G828, October 1999

Role of the vasopressin V1 receptor in regulating the epithelial functions of the guinea pig distal colon

Yoshihiko Sato1, Hiroyuki Hanai2, Atsuhiro Nogaki1, Koki Hirasawa1, Eizo Kaneko1, Hisayoshi Hayashi3, and Yuichi Suzuki3

1 First Department of Medicine, 2 Department of Endoscopic and Photodynamic Medicine, Hamamatsu University School of Medicine, Hamamatsu 431-3192; and 3 Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan

Vasopressin has a wide spectrum of biological action. In this study, the role of vasopressin in regulating electrolyte transport in the colon was elucidated by measuring the short-circuit current (Isc) as well as the Na+, K+, and Cl- flux in a chamber-mounted mucosal sheet. The cytosolic Ca2+ concentration ([Ca2+]i) was also measured in fura 2-loaded cells by fluorescence imaging. Serosal vasopressin decreased Isc at 10-9 M and increased Isc at 10-7-10-6 M. The decrease in Isc was accompanied by two effects: one was a decrease in the amiloride-sensitive Na+ absorption, whereas the other was an increase in the bumetanide-sensitive K+ secretion. The increase in Isc was accompanied by an increase in the Cl- secretion that can be inhibited by serosal bumetanide or mucosal diphenylamine-2-carboxylate. Vasopressin caused an increase in [Ca2+]i in crypt cells. These responses of Isc and the [Ca2+]i increase in crypt cells were all more potently inhibited by the vasopressin V1 receptor antagonist than by the V2 receptor antagonist. These results suggest that vasopressin inhibits electrogenic Na+ absorption and stimulates electrogenic K+ and Cl- secretion. In all of these responses, the V1 receptor is involved, and the [Ca2+]i increase may play an important role.

sodium absorption; potassium secretion; chloride secretion; intracellular calcium; intestinal transport


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