| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
2 Laboratory of Cellular Oncology, Signal Transduction Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892; and 1 Division of Gastroenterology, Research Institute, The Hospital for Sick Children, Departments of Paediatrics and Biochemistry, University of Toronto, Toronto, Ontario, Canada M5G 1X8
Treatment of HT-29 cells with phorbol
12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC),
induces MUC2 expression. To investigate the role of PKC in
regulating mucin genes in intestinal cells, we examined the regulation
of MUC1, MUC2, MUC5AC, MUC5B, and MUC6 expression in two human
mucin-producing colonic cell lines, T84 and HT29/A1. T84 and HT29/A1
cells (at 80-90% confluency) were exposed to 100 nM PMA for 0, 3, and 6 h. Twofold or greater increases in mRNA levels for MUC2 and
MUC5AC were observed in both cell lines during this time period,
whereas the levels of MUC1, MUC5B, and MUC6 mRNAs were only marginally affected. These results indicated that PKC differentially
regulates mucin gene expression and that it may be responsible for
altered mucin expression. Our previous results suggested that the
Ca2+-independent PKC-
isoform
appeared to mediate PMA-regulated mucin exocytosis in these cell lines.
To determine if PKC- was also involved in MUC2/MUC5AC gene
induction, HT29/A1 cells were stably transfected with either a
wild-type PKC- or a dominant-negative ATP-binding mutant of PKC-
(PKC- K437R). Overexpression of the dominant-negative PKC- K437R
blocked induction of both mucin genes, whereas PMA-induced mucin gene
expression was not prevented by overexpression of wild-type PKC-.
PMA-dependent MUC2 mucin secretion was also blocked in cells
overexpressing the dominant-negative PKC- K437R. On the basis of
these observations, PKC- appears to mediate the expression of two
major gastrointestinal mucins in response to PMA as well as
PMA-regulated mucin exocytosis.
protein kinase C; signal transduction; phorbol 12-myristate 13-acetate
This article has been cited by other articles:
|
|
 |
|
  T. A. Wyatt, R. E. Slager, J. DeVasure, B. W. Auvermann, M. L. Mulhern, S. Von Essen, T. Mathisen, A. A. Floreani, and D. J. Romberger Feedlot dust stimulation of interleukin-6 and -8 requires protein kinase C{varepsilon} in human bronchial epithelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1163 - L1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ehre, Y. Zhu, L. H. Abdullah, J. Olsen, K. I. Nakayama, K. Nakayama, R. O. Messing, and C. W. Davis nPKC{varepsilon}, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1445 - C1454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Broughman, L. Sun, S. Umar, J. Scott, J. H. Sellin, and A. P. Morris Chronic PKC-beta activation in HT-29 Cl.19a colonocytes prevents cAMP-mediated ion secretion by inhibiting apical membrane current generation Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G318 - G330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Plaisancie, R. Ducroc, M. E. Homsi, A. Tsocas, S. Guilmeau, S. Zoghbi, O. Thibaudeau, and A. Bado Luminal leptin activates mucin-secreting goblet cells in the large bowel Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G805 - G812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zen, K. Harada, M. Sasaki, K. Tsuneyama, K. Katayanagi, Y. Yamamoto, and Y. Nakanuma Lipopolysaccharide Induces Overexpression of MUC2 and MUC5AC in Cultured Biliary Epithelial Cells : Possible Key Phenomenon of Hepatolithiasis Am. J. Pathol., October 1, 2002; 161(4): 1475 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Braz, O. F. Bueno, L. J. De Windt, and J. D. Molkentin PKC{alpha} regulates the hypertrophic growth of cardiomyocytes through extracellular signal-regulated kinase1/2 (ERK1/2) J. Cell Biol., March 4, 2002; (2002) 200108062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Adler and Y. Li Airway Epithelium and Mucus . Intracellular Signaling Pathways for Gene Expression and Secretion Am. J. Respir. Cell Mol. Biol., October 1, 2001; 25(4): 397 - 400. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Deplancke and H R. Gaskins Microbial modulation of innate defense: goblet cells and the intestinal mucus layer Am. J. Clinical Nutrition, June 1, 2001; 73 (6): 1131S - 1141S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Perrais, P. Pigny, M.-P. Buisine, N. Porchet, J.-P. Aubert, and I. Van Seuningen-Lempire Aberrant Expression of Human Mucin Gene MUC5B in Gastric Carcinoma and Cancer Cells. IDENTIFICATION AND REGULATION OF A DISTAL PROMOTER J. Biol. Chem., April 27, 2001; 276(18): 15386 - 15396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Braz, O. F. Bueno, L. J. De Windt, and J. D. Molkentin PKC{alpha} regulates the hypertrophic growth of cardiomyocytes through extracellular signal-regulated kinase1/2 (ERK1/2) J. Cell Biol., March 4, 2002; 156(5): 905 - 919. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
