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Am J Physiol Gastrointest Liver Physiol 277: G1055-G1063, 1999;
0193-1857/99 $5.00
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Vol. 277, Issue 5, G1055-G1063, November 1999

Expression of voltage-dependent K+ channel genes in mesenteric artery smooth muscle cells

Chuanli Xu, Yanjie Lu, Guanghua Tang, and Rui Wang

Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5

Molecular basis of native voltage-dependent K+ (Kv) channels in smooth muscle cells (SMCs) from rat mesenteric arteries was investigated. The whole cell patch-clamp study revealed that a 4-aminopyridine-sensitive delayed rectifier K+ current (IK) was the predominant K+ conductance in these cells. A systematic screening of the expression of 18 Kv channel genes using RT-PCR technique showed that six IK-encoding genes (Kv1.2, Kv1.3, Kv1.5, Kv2.1, Kv2.2, and Kv3.2) were expressed in mesenteric artery. Although no transient outward Kv currents (IA) were recorded in the studied SMCs, transcripts of multiple IA-encoding genes, including Kv1.4, Kv3.3, Kv3.4, Kv4.1, Kv4.2, and Kv4.3 as well as IA-facilitating Kv beta -subunits (Kvbeta 1, Kvbeta 2, and Kvbeta 3), were detected in mesenteric arteries. Western blot analysis demonstrated that four IK-related Kv channel proteins (Kv1.2, Kv1.3, Kv1.5, and Kv2.1) were detected in mesenteric artery tissues. The presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 channel proteins in isolated SMCs was further confirmed by immunocytochemistry study. Our results suggest that the native IK in rat mesenteric artery SMCs might be generated by heteromultimerization of Kv genes.

Kv channels; peripheral artery; reverse transcription-polymerase chain reaction; Western blot; immunocytochemistry


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