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Am J Physiol Gastrointest Liver Physiol 277: G1180-G1188, 1999;
0193-1857/99 $5.00
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Vol. 277, Issue 6, G1180-G1188, December 1999

Capsaicin sensitivity and voltage-gated sodium currents in colon sensory neurons from rat dorsal root ganglia

Xin Su1, Ruth E. Wachtel2,3, and G. F. Gebhart1

Departments of 1 Pharmacology and 2 Anesthesia, College of Medicine, University of Iowa, Iowa City 52242; and 3 Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246

DiI-labeled colon sensory neurons were acutely dissociated from S1 rat dorsal root ganglia (DRG) and studied using perforated whole cell patch-clamp techniques. Forty-six percent (54/116) of labeled sensory neurons responded to capsaicin (10-8- 10-5 M) with an increase in inward current, which was a nonspecific cation conductance. Responses to capsaicin applied by puffer ejection were dependent on dose, with a half-maximal response at 4.9 × 10-7 M; bath application was characterized by marked desensitization. Voltage-gated Na+ currents in 23 of 30 DRG cells exhibited both TTX-sensitive and TTX-resistant components. In these cells, capsaicin induced an inward current in 11 of 17 cells tested. Of the cells containing only a TTX-sensitive component, none of six cells tested was sensitive to capsaicin. In all cells that responded to capsaicin with an increase in inward current, capsaicin abolished voltage-gated Na+ currents (n = 21). Capsazepine (10-6 M) significantly attenuated both the increase in inward current and the reduction in Na+ currents. Na+ currents were not significantly altered by adenosine, bradykinin, histamine, PGE2, or serotonin at 10-6 M and 10-5 M. These findings may have important implications for understanding both the irritant and analgesic properties of capsaicin.

visceral pain; bradykinin; prostaglandin E2; serotonin; tetrodotoxin


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