AJP - GI Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 277: G1288-G1296, 1999;
0193-1857/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roussa, E.
Right arrow Articles by Thévenod, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roussa, E.
Right arrow Articles by Thévenod, F.
Vol. 277, Issue 6, G1288-G1296, December 1999

Immunolocalization of anion exchanger AE2 and Na+-HCOminus 3 cotransporter in rat parotid and submandibular glands

Eleni Roussa1, Michael F. Romero2, Bernhard M. Schmitt3, Walter F. Boron3, Seth L. Alper4, and Frank Thévenod1

1 Departments of Anatomy and 5 Physiology, Medical Faculty, University of Saarland, 66421 Homburg/Saar, Germany; 2 Department of Physiology and Biophysics and Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4790; 3 Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520; and 4 Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02215

Salivary glands secrete K+ and HCO-3 and reabsorb Na+ and Cl-, but the identity of transporters involved in HCO-3 transport remains unclear. We investigated localization of Cl-/HCO-3 exchanger isoform AE2 and of Na+-HCO-3 cotransporter (NBC) in rat parotid gland (PAR) and submandibular gland (SMG) by immunoblot and immunocytochemical techniques. Immunoblotting of PAR and SMG plasma membranes with specific antibodies against mouse kidney AE2 and rat kidney NBC revealed protein bands at ~160 and 180 kDa for AE2 and ~130 kDa for NBC, as expected for the AE2 full-length protein and consistent with the apparent molecular mass of NBC in several tissues other than kidney. Immunostaining of fixed PAR and SMG tissue sections revealed specific basolateral staining of PAR acinar cells for AE2 and NBC, but in SMG acinar cells only basolateral AE2 labeling was observed. No AE2 expression was detected in any ducts. Striated, intralobular, and main duct cells of both glands showed NBC expression predominantly at basolateral membranes, with some cells being apically stained. In SMG duct cells, NBC staining exhibited a gradient of distribution from basolateral localization in more proximal parts of the ductal tree to apical localization toward distal parts of the ductal tree. Both immunoblotting signals and immunostaining were abolished in preabsorption experiments with the respective antigens. Thus the mechanisms of fluid and anion secretion in salivary acinar cells may be different between PAR and SMG, and, because NBC was detected in acinar and duct cells, it may play a more important role in transport of HCO-3 by rat salivary duct cells than previously believed.

bicarbonate transport; ducts; acini; secretion


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. R. Sussman, J. Zhao, C. Plata, J. Lu, C. Daly, N. Angle, J. DiPiero, I. A. Drummond, J. O. Liang, W. F. Boron, et al.
Cloning, localization, and functional expression of the electrogenic Na+ bicarbonate cotransporter (NBCe1) from zebrafish
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C865 - C875.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Perry, D. O. Quissell, M. E. Reyland, and I. I. Grichtchenko
Electrogenic NBCe1 (SLC4A4), but not electroneutral NBCn1 (SLC4A7), cotransporter undergoes cholinergic-stimulated endocytosis in salivary ParC5 cells
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1385 - C1398.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Heitzmann and R. Warth
Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia
Physiol Rev, July 1, 2008; 88(3): 1119 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Brandes, O. Oehlke, A. Schumann, S. Heidrich, F. Thevenod, and E. Roussa
Adaptive redistribution of NBCe1-A and NBCe1-B in rat kidney proximal tubule and striated ducts of salivary glands during acid-base disturbances
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2400 - R2411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. C. Li, E. Y. Li, L. Neumeier, L. Conforti, and M. Soleimani
Identification of a novel signal in the cytoplasmic tail of the Na+:HCO3- cotransporter NBC1 that mediates basolateral targeting
Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1245 - F1255.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Li, N.-Y. Koo, I.-H. Cho, T.-H. Kwon, S.-Y. Choi, S. J. Lee, S. B. Oh, J.-S. Kim, and K. Park
Expression of the Na+-HCO3- cotransporter and its role in pHi regulation in guinea pig salivary glands
Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1031 - G1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Pushkin and I. Kurtz
SLC4 base (HCO3-, CO32-) transporters: classification, function, structure, genetic diseases, and knockout models
Am J Physiol Renal Physiol, March 1, 2006; 290(3): F580 - F599.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Abdulnour-Nakhoul, N. L. Nakhoul, S. A. Wheeler, P. Wang, E. R. Swenson, and R. C. Orlando
HCO3- secretion in the esophageal submucosal glands
Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G736 - G744.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Abuladze, A. Pushkin, S. Tatishchev, D. Newman, P. Sassani, and I. Kurtz
Expression and localization of rat NBC4c in liver and renal uroepithelium
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C781 - C789.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. P. J. Diecke, Q. Wen, J. M. Sanchez, K. Kuang, and J. Fischbarg
Immunocytochemical localization of Na+-HCO3- cotransporters and carbonic anhydrase dependence of fluid transport in corneal endothelial cells
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1434 - C1442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H.-V. Nguyen, A. Stuart-Tilley, S. L. Alper, and J. E. Melvin
Cl-/HCO3- exchange is acetazolamide sensitive and activated by a muscarinic receptor-induced [Ca2+]i increase in salivary acinar cells
Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G312 - G320.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Choi, L. Hu, J. D. Rojas, B. M. Schmitt, and W. F. Boron
Role of glycosylation in the renal electrogenic Na+-HCO-3 cotransporter (NBCe1)
Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1199 - F1206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Satoh, N. Moriyama, C. Hara, H. Yamada, S. Horita, M. Kunimi, K. Tsukamoto, N. Iso-o, J. Inatomi, H. Kawakami, et al.
Localization of Na+-HCO-3 cotransporter (NBC-1) variants in rat and human pancreas
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C729 - C737.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Kulaksiz, E. Rehberg, W. Stremmel, and Y. Cetin
Guanylin and Functional Coupling Proteins in the Human Salivary Glands and Gland Tumors : Expression, Cellular Localization, and Target Membrane Domains
Am. J. Pathol., August 1, 2002; 161(2): 655 - 664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
V. Gresz, T.-H. Kwon, H. Vorum, T. Zelles, I. Kurtz, M. C. Steward, C. Aalkjaer, and S. Nielsen
Immunolocalization of electroneutral Na+-HCO3- cotransporters in human and rat salivary glands
Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G473 - G480.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Roussa, S. L. Alper, and F. Thévenod
Immunolocalization of Anion Exchanger AE2, Na+/H+ Exchangers NHE1 and NHE4, and Vacuolar Type H+-ATPase in Rat Pancreas
J. Histochem. Cytochem., April 1, 2001; 49(4): 463 - 474.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
A. B. MAUNSBACH, H. VORUM, T.-H. KWON, S. NIELSEN, B. SIMONSEN, I. CHOI, B. M. SCHMITT, W. F. BORON, and C. AALKJæR
Immunoelectron Microscopic Localization of the Electrogenic Na/HCO3 Cotransporter in Rat and Ambystoma Kidney
J. Am. Soc. Nephrol., December 1, 2000; 11(12): 2179 - 2189.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online