| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Clinical Sciences, 2 Food Animal Health and Resource Management, and 3 Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh, 27606; and 4 Department of Pediatrics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599.
We have previously shown that PGE2 enhances
recovery of transmucosal resistance (R) in
ischemia-injured porcine ileum via a mechanism involving
chloride secretion. Because the tyrosine kinase inhibitor genistein
amplifies cAMP-induced Cl
secretion, we postulated
that genistein would augment PGE2-induced recovery of
R. Porcine ileum subjected to 45 min of ischemia was mounted in Ussing chambers, and R and mucosal-to-serosal fluxes of [3H]N-formyl-methionyl-leucyl
phenylalanine (FMLP) and [3H]mannitol were
monitored as indicators of recovery of barrier function. Treatment with
genistein (104 M) and PGE2
(106 M) resulted in synergistic
elevations in R and additive reductions in mucosal-to-serosal
fluxes of [3H]FMLP and
[3H]mannitol, whereas treatment with genistein
alone had no effect. Treatment of injured tissues with genistein and
either 8-bromo-cAMP (104 M) or cGMP
(104 M) resulted in synergistic
increases in R. However, treatment of tissues with genistein
and the protein kinase C (PKC) agonist phorbol myristate acetate
(105-106
M) had no effect on R. Genistein augments recovery of R
in the presence of cAMP or cGMP but not in the presence of PKC agonists.
mucosa; chloride secretion; transmucosal resistance; tyrosine kinase
This article has been cited by other articles:
|
|
 |
|
  A. T. Blikslager, A. J. Moeser, J. L. Gookin, S. L. Jones, and J. Odle Restoration of Barrier Function in Injured Intestinal Mucosa Physiol Rev, April 1, 2007; 87(2): 545 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Rhoads, B. A. Corl, R. Harrell, X. Niu, L. Gatlin, O. Phillips, A. Blikslager, A. Moeser, G. Wu, and J. Odle Intestinal ribosomal p70S6K signaling is increased in piglet rotavirus enteritis Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G913 - G922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Moeser, P. K. Nighot, K. J. Engelke, R. Ueno, and A. T. Blikslager Recovery of mucosal barrier function in ischemic porcine ileum and colon is stimulated by a novel agonist of the ClC-2 chloride channel, lubiprostone Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G647 - G656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Campbell, C. G. Ruaux, D. E. Shifflett, J. M. Steiner, D. A. Williams, and A. T. Blikslager Physiological concentrations of bile salts inhibit recovery of ischemic-injured porcine ileum Am J Physiol Gastrointest Liver Physiol, August 1, 2004; 287(2): G399 - G407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Shifflett, S. L. Jones, A. J. Moeser, and A. T. Blikslager Mitogen-activated protein kinases regulate COX-2 and mucosal recovery in ischemic-injured porcine ileum Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G906 - G913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Little, R. A. Dean, K. M. Young, S. A. McKane, L. D. Martin, S. L. Jones, and A. T. Blikslager PI3K signaling is required for prostaglandin-induced mucosal recovery in ischemia-injured porcine ileum Am J Physiol Gastrointest Liver Physiol, January 1, 2003; 284(1): G46 - G56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A T Blikslager, D N Zimmel, K M Young, N B Campbell, D Little, and R A Argenzio Recovery of ischaemic injured porcine ileum: evidence for a contributory role of COX-1 and COX-2 Gut, May 1, 2002; 50(5): 615 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Blikslager, S. M. Pell, and K. M. Young PGE2 triggers recovery of transmucosal resistance via EP receptor cross talk in porcine ischemia-injured ileum Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G375 - G381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. McKay, F. Botelho, P. J. M. Ceponis, and C. D. Richards Superantigen immune stimulation activates epithelial STAT-1 and PI 3-K: PI 3-K regulation of permeability Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G1094 - G1103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Lawrence, K. M. Comerford, and S. P. Colgan Role of VASP in reestablishment of epithelial tight junction assembly after Ca2+ switch Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1235 - C1245. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
