AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G259-G265, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (43)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galeazzi, F.
Right arrow Articles by Collins, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galeazzi, F.
Right arrow Articles by Collins, S. M.
Vol. 278, Issue 2, G259-G265, February 2000

Inflammation-induced impairment of enteric nerve function in nematode-infected mice is macrophage dependent

Francesca Galeazzi1, Eric M. Haapala1, Nico van Rooijen3, Bruce A. Vallance1, and Stephen M. Collins1,2

1 Intestinal Diseases Research Programme and 2 Gastrointestinal Division and Department of Medicine, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and 3 Department of Cell Biology, Vriej Universiteit, Amsterdam, The Netherlands

Trichinella spiralis infection in rodents is associated with suppression of ACh release from myenteric plexus that can be mimicked by macrophage-derived cytokines. We verified the presence of a macrophage infiltrate in the intestine during T. spiralis infection and determined the extent to which this cell type is responsible for the neural changes. C57BL/6 mice were infected with 375 T. spiralis larvae by gavage, and the presence of macrophages (F4/80 positive) in the jejunum was determined immunohistochemically. In another experiment, infected mice were treated intravenously with liposomes containing dichloromethylene diphosphonate (clodronate, Cl2MDP), which causes apoptosis of macrophages, and killed at postinfection day 6, and jejunal tissues were evaluated for the presence of F4/80-positive cells and for [3H]ACh release from the myenteric plexus. Infection caused an infiltration of F4/80-positive cells into the intestinal mucosa, muscle layers, and myenteric plexus region and a significant suppression of ACh release (50%). Depletion of F4/80-positive macrophages using Cl2MDP-containing liposomes prevented the suppression in [3H]ACh release, identifying macrophages as the cell type involved in the functional impairment of enteric cholinergic nerves.

inflammatory bowel disease; enteric nervous system; acetylcholine; liposomes


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J.-E. Ghia, P. Blennerhassett, R. T. El-Sharkawy, and S. M. Collins
The protective effect of the vagus nerve in a murine model of chronic relapsing colitis
Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G711 - G718.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Cheng, W. Cao, C. Fiocchi, J. Behar, P. Biancani, and K. M. Harnett
Platelet-activating factor and prostaglandin E2 impair esophageal ACh release in experimental esophagitis
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G418 - G428.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. Cao, L. Cheng, J. Behar, C. Fiocchi, P. Biancani, and K. M. Harnett
Proinflammatory cytokines alter/reduce esophageal circular muscle contraction in experimental cat esophagitis
Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1131 - G1139.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Overhaus, S. Togel, M. A. Pezzone, and A. J. Bauer
Mechanisms of polymicrobial sepsis-induced ileus
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G685 - G694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Suzuki, K.-J. Won, K. Horiguchi, K. Kinoshita, M. Hori, S. Torihashi, E. Momotani, K. Itoh, K. Hirayama, S. M. Ward, et al.
Muscularis inflammation and the loss of interstitial cells of Cajal in the endothelin ETB receptor null rat
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G638 - G646.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. L. Green, W. Ho, K. A. Sharkey, and D. M. McKay
Dextran sodium sulfate-induced colitis reveals nicotinic modulation of ion transport via iNOS-derived NO
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G706 - G714.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
S M COLLINS, T PICHE, and P RAMPAL
The putative role of inflammation in the irritable bowel syndrome
Gut, December 1, 2001; 49(6): 743 - 745.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. I. Khan, P. A. Blennerhassett, Y. Deng, J. Gauldie, B. A. Vallance, and S. M. Collins
IL-12 gene transfer alters gut physiology and host immunity in nematode-infected mice
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G102 - G110.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
F. Galeazzi, P. Lovato, P. A. Blennerhassett, E. M. Haapala, B. A. Vallance, and S. M. Collins
Neural change in Trichinella-infected mice is MHC II independent and involves M-CSF-derived macrophages
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G151 - G158.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online