AJP - GI Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G308-G320, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan, S.
Right arrow Articles by Hooi, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan, S.
Right arrow Articles by Hooi, S. C.
Vol. 278, Issue 2, G308-G320, February 2000

Syncollin is differentially expressed in rat proximal small intestine and regulated by feeding behavior

Sandra Tan and Shing C. Hooi

Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore 119260

Gradients of gene expression are maintained along the proximal-distal axis of the mammalian small intestine despite a continuously regenerating epithelium. To study the molecular mechanisms responsible for this phenomenon, we utilized a subtractive hybridization strategy to isolate genes differentially expressed in the duodenum but not ileum. We isolated and sequenced 15 clones. The clones were fragments of genes encoding lipases, proteases, and an esterase. A novel clone was characterized and subsequently shown to encode syncollin, a secretory granule protein that binds to syntaxin in a calcium-sensitive manner. RT-PCR and S1 nuclease protection assay were used to clarify the 5'-end of syncollin. Syncollin was expressed in the rat pancreas, spleen, duodenum, and colon. In situ hybridization localized syncollin expression in the pancreas to acinar cells and in the duodenum to villus epithelial cells.

subtractive hybridization; duodenum-specific genes


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
J.-P. Bach, H. Borta, W. Ackermann, F. Faust, O. Borchers, and M. Schrader
The Secretory Granule Protein Syncollin Localizes to HL-60 Cells and Neutrophils
J. Histochem. Cytochem., August 1, 2006; 54(8): 877 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
L B Hays, B Wicksteed, Y Wang, J F McCuaig, L H Philipson, J M Edwardson, and C J Rhodes
Intragranular targeting of syncollin, but not a syncollinGFP chimera, inhibits regulated insulin exocytosis in pancreatic {beta}-cells
J. Endocrinol., April 1, 2005; 185(1): 57 - 67.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online