| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 St Mark's Hospital, Harrow HA1 3UJ; 2 Imperial Cancer Research Fund, London WC2A 3PX; and 3 Imperial College School of Medicine, Hammersmith Campus, London W12 0NN, United Kingdom
To determine the effect of glucagon-like peptide-2 (GLP-2) on sucrase-isomaltase and caudal-related homeobox protein-2 (Cdx-2) gene expression, male Wistar rats were divided into total parenteral nutrition (TPN)-fed and GLP-2-treated, TPN-fed groups. TPN was given via a jugular line, inserted under anesthesia, for 7 days. The treatment group received 40 µg/day of GLP-2 intravenously with the TPN diet. The small intestine and colon were weighed and measured. Tissue was obtained from the jejunum, terminal ileum, and midcolon. RNA analysis, morphometry, and microdissection were performed. The weight of the small intestine of GLP-2-treated rats was greater than that of TPN-fed rats (P < 0.001). GLP-2 increased the mean metaphase arrests/crypt in both the jejunum and ileum (P < 0.001). Ileal expression of sucrase-isomaltase was increased by 1.6-fold (P < 0.05). Jejunal expression was increased by a similar amount, although not significantly (P = 0.08). There was no change in Cdx-2 gene expression. Thus GLP-2 can maintain small intestinal morphology and function, but effects on gene expression are not mediated by gross changes in the level of the mRNA for the homeobox protein Cdx-2.
total parenteral nutrition; cellular proliferation; jejunum; ileum.
This article has been cited by other articles:
|
|
 |
|
  M. C. Koopmann, D. W. Nelson, S. G. Murali, X. Liu, M. S. Brownfield, J. J. Holst, and D. M. Ney Exogenous Glucagon-Like Peptide-2 (GLP-2) Augments GLP-2 Receptor mRNA and Maintains Proglucagon mRNA Levels in Resected Rats JPEN J Parenter Enteral Nutr, May 1, 2008; 32(3): 254 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Hare, B. Hartmann, H. Kissow, J. J. Holst, and S. S. Poulsen The Intestinotrophic Peptide, GLP-2, Counteracts Intestinal Atrophy in Mice Induced by the Epidermal Growth Factor Receptor Inhibitor, Gefitinib Clin. Cancer Res., September 1, 2007; 13(17): 5170 - 5175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. BRUBAKER The Glucagon-Like Peptides: Pleiotropic Regulators of Nutrient Homeostasis Ann. N.Y. Acad. Sci., July 1, 2006; 1070(1): 10 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. Petersen, D. G. Burrin, and P. T. Sangild GLP-2 has differential effects on small intestine growth and function in fetal and neonatal pigs Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2001; 281(6): R1986 - R1993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Burrin, Y. Petersen, B. Stoll, and P. Sangild Glucagon-Like Peptide 2: A Nutrient-Responsive Gut Growth Factor J. Nutr., March 1, 2001; 131(3): 709 - 712. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. D. Mottino, T. Hoffman, P. A. Dawson, M. G. Luquita, J. A. Monti, E. J. Sanchez Pozzi, V. A. Catania, J. Cao, and M. Vore Increased expression of ileal apical sodium-dependent bile acid transporter in postpartum rats Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G41 - G50. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
