AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G467-G476, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kono, H.
Right arrow Articles by Thurman, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kono, H.
Right arrow Articles by Thurman, R. G.
Vol. 278, Issue 3, G467-G476, March 2000

Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol

Hiroshi Kono1, Nobuyuki Enomoto1, Henry D. Connor1, Michael D. Wheeler1, Blair U. Bradford1, Chantal A. Rivera1, Maria B. Kadiiska2, Ronald P. Mason2, and Ronald G. Thurman1

1 Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill 27599-7365; and 2 Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

This study determined whether free radical formation by the liver, tumor necrosis factor (TNF)-alpha production by isolated Kupffer cells, and plasma endotoxin are affected by dietary saturated fat. Rats were fed enteral ethanol and corn oil (E-CO) or medium-chain triglycerides (E-MCT) and control rats received corn oil (C-CO) or medium-chain triglycerides (C-MCT) for 2 wk. E-CO rats developed moderate fatty infiltration and slight inflammation; however, E-MCT prevented liver injury. Serum aspartate aminotransferase levels, gut permeability, and plasma endotoxin doubled with E-CO but were blunted ~50% with E-MCT. In Kupffer cells from E-CO rats, intracellular calcium was elevated by lipopolysaccharide (LPS) in a dose-dependent manner. In cells from E-MCT rats, increases were blunted by ~40-50% at all concentrations of LPS. The LPS-induced increase in TNF-alpha production by Kupffer cells was dose dependent and was blunted by 40% by MCT. E-CO increased radical adducts and was reduced ~50% by MCT. MCT prevent early alcohol-induced liver injury, in part, by inhibition of free radical formation and TNF-alpha production by inhibition of endotoxin-mediated activation of Kupffer cells.

tumor necrosis factor-alpha ; intracellular calcium; free radicals; alcohol


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
H. Kono, H. Fujii, Y. Hirai, M. Tsuchiya, H. Amemiya, M. Asakawa, A. Maki, M. Matsuda, and M. Yamamoto
The Kupffer cell protects against acute lung injury in a rat peritonitis model: role of IL-10
J. Leukoc. Biol., April 1, 2006; 79(4): 809 - 817.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
H. P. Glauert, Z. Lu, A. Kumar, R. P. Bunaciu, S. Patel, J. C. Tharappel, D. N. Stemm, H.-J. Lehmler, E. Y. Lee, L. W. Robertson, et al.
Dietary Vitamin E Does Not Inhibit the Promotion of Liver Carcinogenesis by Polychlorinated Biphenyls in Rats
J. Nutr., February 1, 2005; 135(2): 283 - 286.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Kono, H. Fujii, M. Asakawa, A. Maki, H. Amemiya, Y. Hirai, M. Matsuda, and M. Yamamoto
Medium-chain triglycerides enhance secretory IgA expression in rat intestine after administration of endotoxin
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G1081 - G1089.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Kono, M. Nakagami, I. Rusyn, H. D. Connor, B. Stefanovic, D. A. Brenner, R. P. Mason, G. E. Arteel, and R. G. Thurman
Development of an animal model of chronic alcohol-induced pancreatitis in the rat
Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1178 - G1186.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online