AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G625-G634, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hosoda, Y.
Right arrow Articles by Kuwahara, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hosoda, Y.
Right arrow Articles by Kuwahara, A.
Vol. 278, Issue 4, G625-G634, April 2000

Mode of action of ANG II on ion transport in guinea pig distal colon

Yutaka Hosoda1, Adi Winarto2, Toshihiko Iwanaga2, and Atsukazu Kuwahara1

1 Laboratory of Environmental Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526; and 2 Laboratory of Veterinary Anatomy, Hokkaido University, Sapporo 060-0818, Japan

The effect of ANG II on mucosal ion transport and localization of ANG type 1 receptor (AT1R) in the guinea pig distal colon was investigated. Submucosal/mucosal segments were mounted in Ussing flux chambers, and short-circuit current (Isc) was measured as an index of ion transport. Serosal addition of ANG II produced a concentration-dependent (10-9-10-5 M) increase in Isc. The maximal response was observed at 10-6 M; the increase in Isc was 164.4 ± 11.8 µA/cm2. The ANG II (10-6 M)-evoked response was mainly due to Cl- secretion. Tetrodotoxin, atropine, the neurokinin type 1 receptor antagonist FK-888, and piroxicam significantly reduced the ANG II (10-6 M)-evoked response to 28, 45, 58, and 16% of control, respectively. Pretreatment with prostaglandin E2 (10-5 M) resulted in a threefold increase in the ANG II-evoked response. The AT1R antagonist FR-130739 completely blocked ANG II (10-6 M)-evoked responses, whereas the ANG type 2 receptor antagonist PD-123319 had no effect. Localization of AT1R was determined by immunohistochemistry. In the immunohistochemical study, AT1R-immunopositive cells were distributed clearly in enteric nerves and moderately in surface epithelial cells. These results suggest that ANG II-evoked electrogenic Cl- secretion may involve submucosal cholinergic and tachykinergic neurons and prostanoid synthesis pathways through AT1R on the submucosal plexus and surface epithelial cells in guinea pig distal colon.

substance P; prostaglandin; local renin-angiotensin system; angiotensin type 1 receptor; neurokinin type 1 receptor


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. Paul, A. Poyan Mehr, and R. Kreutz
Physiology of local Renin-Angiotensin systems.
Physiol Rev, July 1, 2006; 86(3): 747 - 803.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G.-D. Wang, X.-Y. Wang, H.-Z. Hu, X.-C. Fang, S. Liu, N. Gao, Y. Xia, and J. D. Wood
Angiotensin receptors and actions in guinea pig enteric nervous system
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G614 - G626.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
R. Wu, M.-A. Laplante, and J. de Champlain
Cyclooxygenase-2 Inhibitors Attenuate Angiotensin II-Induced Oxidative Stress, Hypertension, and Cardiac Hypertrophy in Rats
Hypertension, June 1, 2005; 45(6): 1139 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Chiu, C. Santiskulvong, and E. Rozengurt
EGF receptor transactivation mediates ANG II-stimulated mitogenesis in intestinal epithelial cells through the PI3-kinase/Akt/mTOR/p70S6K1 signaling pathway
Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G182 - G194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Hosoda, S.-I. Karaki, Y. Shimoda, and A. Kuwahara
Substance P-evoked Cl- secretion in guinea pig distal colonic epithelia: interaction with PGE2
Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G347 - G356.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
K. Hirasawa, Y. Sato, Y. Hosoda, T. Yamamoto, and H. Hanai
Immunohistochemical Localization of Angiotensin II Receptor and Local Renin-Angiotensin System in Human Colonic Mucosa
J. Histochem. Cytochem., February 1, 2002; 50(2): 275 - 282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. H. Ko, V. W. Y. Law, W. C. Y. Yip, G. G. L. Yue, C. W. Lau, Z. Y. Chen, and Y. Huang
Stimulation of chloride secretion by baicalein in isolated rat distal colon
Am J Physiol Gastrointest Liver Physiol, March 1, 2002; 282(3): G508 - G518.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online