| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Second Department of Pathology, 2 Second Department of Surgery, and 3 Department of Parasitology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692; and 4 Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan
Hepatocyte growth factor (HGF) activator inhibitor type 1 (HAI-1) and type 2 (HAI-2) are new Kunitz-type serine protease inhibitors that were recently purified and cloned from the human stomach cancer cell line MKN45 as specific inhibitors against HGF activator. Both proteins contain two Kunitz inhibitor domains and are expressed abundantly throughout the gastrointestinal tract, in addition to the placenta, pancreas, and kidney. In this study, to assess the possible roles of HAI-1 and HAI-2 in the intestinal mucosa, we examined the expression of HAI-1 and HAI-2 during regeneration of the intestinal mucosa. Immunohistochemical studies revealed that HAI-1 but not HAI-2 was detected more strongly in regenerative epithelium than in normal epithelium, although both proteins were detected throughout the human gastrointestinal tract. During the course of acetic acid-induced experimental colitis in an in vivo mouse model, HAI-1 but not HAI-2 was upregulated in the recovery phase, suggesting that HAI-1 but not HAI-2 is associated with the regeneration of damaged colonic mucosa. Upregulation of HAI-1 may serve to downregulate the proliferative response after initial activation of MET receptor by HGF/scatter factor after an injury.
hepatocyte growth factor; Kunitz-type proteinase inhibitor; mucosal injury; experimental colitis; immunohistochemistry
This article has been cited by other articles:
|
|
 |
|
  K. Kojima, S. Tsuzuki, T. Fushiki, and K. Inouye Roles of Functional and Structural Domains of Hepatocyte Growth Factor Activator Inhibitor Type 1 in the Inhibition of Matriptase J. Biol. Chem., February 1, 2008; 283(5): 2478 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tanaka, K. Nagaike, N. Takeda, H. Itoh, K. Kohama, T. Fukushima, S. Miyata, S. Uchiyama, S. Uchinokura, T. Shimomura, et al. Hepatocyte Growth Factor Activator Inhibitor Type 1 (HAI-1) Is Required for Branching Morphogenesis in the Chorioallantoic Placenta Mol. Cell. Biol., July 1, 2005; 25(13): 5687 - 5698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tahara, A. Ido, S. Yamamoto, Y. Miyata, H. Uto, T. Hori, K. Hayashi, and H. Tsubouchi Hepatocyte Growth Factor Facilitates Colonic Mucosal Repair in Experimental Ulcerative Colitis in Rats J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 146 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kataoka, R. Hamasuna, H. Itoh, N. Kitamura, and M. Koono Activation of Hepatocyte Growth Factor/Scatter Factor in Colorectal Carcinoma Cancer Res., November 1, 2000; 60(21): 6148 - 6159. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kataoka, T. Shimomura, T. Kawaguchi, R. Hamasuna, H. Itoh, N. Kitamura, K. Miyazawa, and M. Koono Hepatocyte Growth Factor Activator Inhibitor Type 1 Is a Specific Cell Surface Binding Protein of Hepatocyte Growth Factor Activator (HGFA) and Regulates HGFA Activity in the Pericellular Microenvironment J. Biol. Chem., December 15, 2000; 275(51): 40453 - 40462. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
