AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G811-G819, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, N. L.
Right arrow Articles by Sherman, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, N. L.
Right arrow Articles by Sherman, P. M.
Vol. 278, Issue 5, G811-G819, May 2000

Escherichia coli Shiga toxins induce apoptosis in epithelial cells that is regulated by the Bcl-2 family

Nicola L. Jones1,2,3, Avinash Islur1, Rizwan Haq4, Mariola Mascarenhas1, Mohamed A. Karmali2, Mary H. Perdue5, Brent W. Zanke4, and Philip M. Sherman1,2,3

1 Research Institute, Hospital for Sick Children, and Departments of 2 Molecular Microbiology and Medical Genetics and 3 Pediatrics, University of Toronto, Toronto M5G 1X8; 4 Department of Medicine, University of Toronto, Princess Margaret Hospital, and Ontario Cancer Institute, Toronto M5G 2M9; and 5 Intestinal Diseases Research Program, McMaster University, Hamilton, Ontario, Canada L8N 3Z5

Human intestinal cells lack globotriaosylceramide (Gb3), the receptor for Shiga toxin-1 (Stx1) and Shiga toxin-2 (Stx2). Therefore, the role of these toxins in mediating intestinal disease during infection with Shiga toxin-producing Escherichia coli is unclear. The aims of this study were to determine whether Stx1 and Stx2 induce apoptosis in epithelial cells expressing (HEp-2, Caco-2) or lacking (T84) Gb3 and to characterize the role of the Bcl-2 family. Stx1 (12.5 ng/ml) induced apoptosis in both HEp-2 (21.9 ± 7.9% vs. 0.8 ± 0.3%, P = 0.01) and Caco-2 (10.1 ± 1.2% vs. 3.1 ± 0.4%, P = 0.006) cells but not in Gb3-deficient T84 cells. Toxin-mediated apoptosis of HEp-2 cells was associated with enhanced expression of the proapoptotic protein Bax. Inhibition of caspase activation prevented toxin-stimulated apoptosis. In addition, overexpression of Bcl-2 by transient transfection blocked Stx1-stimulated cell death. These findings indicate that Shiga toxins produced by E. coli signal Gb3-expressing epithelial cells to undergo apoptosis in association with enhanced Bax expression, thereby resulting in activation of the caspase cascade.

caspases; Bax; programmed cell death


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. Fujii, K. Wood, F. Matsuda, B. A. Carneiro-Filho, K. H. Schlegel, T. Yutsudo, B. Binnington-Boyd, C. A. Lingwood, F. Obata, K. S. Kim, et al.
Shiga Toxin 2 Causes Apoptosis in Human Brain Microvascular Endothelial Cells via C/EBP Homologous Protein
Infect. Immun., August 1, 2008; 76(8): 3679 - 3689.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
T. Falguieres, M. Maak, C. von Weyhern, M. Sarr, X. Sastre, M.-F. Poupon, S. Robine, L. Johannes, and K.-P. Janssen
Human colorectal tumors and metastases express Gb3 and can be targeted by an intestinal pathogen-based delivery tool
Mol. Cancer Ther., August 1, 2008; 7(8): 2498 - 2508.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. S. Frick, K. Fink, F. Kahl, M. J. Niemiec, M. Quitadamo, K. Schenk, and I. B. Autenrieth
Identification of Commensal Bacterial Strains That Modulate Yersinia enterocolitica and Dextran Sodium Sulfate-Induced Inflammatory Responses: Implications for the Development of Probiotics
Infect. Immun., July 1, 2007; 75(7): 3490 - 3497.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S.-Y. Lee, R. P. Cherla, and V. L. Tesh
Simultaneous Induction of Apoptotic and Survival Signaling Pathways in Macrophage-Like THP-1 Cells by Shiga Toxin 1
Infect. Immun., March 1, 2007; 75(3): 1291 - 1302.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Grishin, H. Ford, J. Wang, H. Li, V. Salvador-Recatala, E. S. Levitan, and E. Zaks-Makhina
Attenuation of apoptosis in enterocytes by blockade of potassium channels
Am J Physiol Gastrointest Liver Physiol, November 1, 2005; 289(5): G815 - G821.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Nakayama, R. G. Pergolizzi, and R. G. Crystal
Gene Transfer-Mediated Pre-mRNA Segmental Trans-splicing As a Strategy to Deliver Intracellular Toxins for Cancer Therapy
Cancer Res., January 1, 2005; 65(1): 254 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y. Wu, B. Lau, S. Smith, K. Troyan, and D. E. Barnett Foster
Enteropathogenic Escherichia coli Infection Triggers Host Phospholipid Metabolism Perturbations
Infect. Immun., December 1, 2004; 72(12): 6764 - 6772.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. T. Green, M. Lyte, C. Chen, Y. Xie, M. A. Casey, A. Kulkarni-Narla, L. Vulchanova, and D. R. Brown
Adrenergic modulation of Escherichia coli O157:H7 adherence to the colonic mucosa
Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1238 - G1246.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. Yilmaz, T. Jungas, P. Verbeke, and D. M. Ojcius
Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway Contributes to Survival of Primary Epithelial Cells Infected with the Periodontal Pathogen Porphyromonas gingivalis
Infect. Immun., July 1, 2004; 72(7): 3743 - 3751.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. J. Menaker, P. J. M. Ceponis, and N. L. Jones
Helicobacter pylori Induces Apoptosis of Macrophages in Association with Alterations in the Mitochondrial Pathway
Infect. Immun., May 1, 2004; 72(5): 2889 - 2898.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Fujii, T. Matsui, D. P. Heatherly, K. H. Schlegel, P. I. Lobo, T. Yutsudo, G. M. Ciraolo, R. E. Morris, and T. Obrig
Rapid Apoptosis Induced by Shiga Toxin in HeLa Cells
Infect. Immun., May 1, 2003; 71(5): 2724 - 2735.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. J. M. Ceponis, D. M. McKay, J. C. Y. Ching, P. Pereira, and P. M. Sherman
Enterohemorrhagic Escherichia coli O157:H7 Disrupts Stat1-Mediated Gamma Interferon Signal Transduction in Epithelial Cells
Infect. Immun., March 1, 2003; 71(3): 1396 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
W. E. Smith, A. V. Kane, S. T. Campbell, D. W. K. Acheson, B. H. Cochran, and C. M. Thorpe
Shiga Toxin 1 Triggers a Ribotoxic Stress Response Leading to p38 and JNK Activation and Induction of Apoptosis in Intestinal Epithelial Cells
Infect. Immun., March 1, 2003; 71(3): 1497 - 1504.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Cameron, D. Bingham, A. Paul, M. Pavelka, S. Cameron, D. Rotondo, and R. Plevin
Essential Role for Verotoxin in Sustained Stress-Activated Protein Kinase and Nuclear Factor Kappa B Signaling, Stimulated by Escherichia coli O157:H7 in Vero Cells
Infect. Immun., October 1, 2002; 70(10): 5370 - 5380.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. C. Y. Ching, N. L. Jones, P. J. M. Ceponis, M. A. Karmali, and P. M. Sherman
Escherichia coli Shiga-Like Toxins Induce Apoptosis and Cleavage of Poly(ADP-Ribose) Polymerase via In Vitro Activation of Caspases
Infect. Immun., August 1, 2002; 70(8): 4669 - 4677.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Abul-Milh, Y. Wu, B. Lau, C. A. Lingwood, and D. B. Foster
Induction of Epithelial Cell Death Including Apoptosis by Enteropathogenic Escherichia coli Expressing Bundle-Forming Pili
Infect. Immun., December 1, 2001; 69(12): 7356 - 7364.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Shibayama, Y. Doi, N. Shibata, T. Yagi, T. Nada, Y. Iinuma, and Y. Arakawa
Apoptotic Signaling Pathway Activated by Helicobacter pylori Infection and Increase of Apoptosis-Inducing Activity under Serum-Starved Conditions
Infect. Immun., May 1, 2001; 69(5): 3181 - 3189.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online