AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 278: G974-G980, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murthy, K. S.
Right arrow Articles by Makhlouf, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murthy, K. S.
Right arrow Articles by Makhlouf, G. M.
Vol. 278, Issue 6, G974-G980, June 2000

Gi-1/Gi-2-dependent signaling by single-transmembrane natriuretic peptide clearance receptor

K. S. Murthy, B.-Q. Teng, H. Zhou, J.-G. Jin, J. R. Grider, and G. M. Makhlouf

Departments of Physiology and Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298-0711

Single-transmembrane natriuretic peptide clearance receptor (NPR-C), which is devoid of a cytoplasmic guanylyl cyclase domain, interacts with pertussis toxin (PTx)-sensitive G proteins to activate endothelial nitric oxide synthase (eNOS) expressed in gastrointestinal smooth muscle cells. We examined the ability of NPR-C to activate other effector enzymes in eNOS-deficient tenia coli smooth muscle cells; these cells expressed NPR-C and NPR-B but not NPR-A. Atrial natriuretic peptide (ANP), the selective NPR-C ligand cANP-(4-23), and vasoactive intestinal peptide (VIP) inhibited 125I-ANP and 125I-VIP binding to muscle membranes in a pattern indicating high-affinity binding to NPR-C. Interaction of VIP with NPR-C was confirmed by its ability to inhibit 125I-ANP binding to membranes of NPR-C-transfected COS-1 cells. In tenia muscle cells, all ligands selectively activated Gi-1 and Gi-2; VIP also activated Gs via VIP2 receptors. All ligands stimulated phosphoinositide hydrolysis, which was inhibited by ANP-(1-11), PTx, and antibodies to phospholipase C-beta 3 (PLC-beta 3) and Gbeta . cANP-(4-23) contracted tenia muscle cells; contraction was blocked by U-73122 and PTx and by antibodies to PLC-beta 3 and Gbeta in intact and permeabilized muscle cells, respectively. VIP and ANP contracted muscle cells only after inhibition of cAMP- and cGMP-dependent protein kinases. ANP and cANP-(4-23) inhibited forskolin-stimulated cAMP in a PTx-sensitive fashion. We conclude that NPR-C is coupled to activation of PLC-beta 3 via beta gamma -subunits of Gi-1 and Gi-2 and to inhibition of adenylyl cyclase via alpha -subunits.

G protein; smooth muscle; vasoactive intestinal peptide; signal transduction; phosphoinositide metabolism; phospholipase C


This article has been cited by other articles:


Home page
 J. Physiol.Home page
R. A. Rose and W. R. Giles
Natriuretic peptide C receptor signalling in the heart and vasculature
J. Physiol., January 15, 2008; 586(2): 353 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. E. Sabbatini, M. Rodriguez, M. B. di Carlo, C. A. Davio, M. S. Vatta, and L. G. Bianciotti
C-type natriuretic peptide enhances amylase release through NPR-C receptors in the exocrine pancreas
Am J Physiol Gastrointest Liver Physiol, November 1, 2007; 293(5): G987 - G994.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. A. Rose, N. Hatano, S. Ohya, Y. Imaizumi, and W. R. Giles
C-type natriuretic peptide activates a non-selective cation current in acutely isolated rat cardiac fibroblasts via natriuretic peptide C receptor-mediated signalling
J. Physiol., April 1, 2007; 580(1): 255 - 274.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. E. Sabbatini, M. S. Vatta, C. A. Davio, and L. G. Bianciotti
Atrial natriuretic factor negatively modulates secretin intracellular signaling in the exocrine pancreas
Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G349 - G357.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
G. Singh, R. E. Kuc, J. J. Maguire, M. Fidock, and A. P. Davenport
Novel Snake Venom Ligand Dendroaspis Natriuretic Peptide Is Selective for Natriuretic Peptide Receptor-A in Human Heart: Downregulation of Natriuretic Peptide Receptor-A in Heart Failure
Circ. Res., July 21, 2006; 99(2): 183 - 190.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. A. Rose, M. B. Anand-Srivastava, W. R. Giles, and J. S. Bains
C-type Natriuretic Peptide Inhibits L-type Ca2+ Current in Rat Magnocellular Neurosecretory Cells by Activating the NPR-C Receptor
J Neurophysiol, July 1, 2005; 94(1): 612 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Furst, C. Brueckl, W. M. Kuebler, S. Zahler, F. Krotz, A. Gorlach, A. M. Vollmar, and A. K. Kiemer
Atrial Natriuretic Peptide Induces Mitogen-Activated Protein Kinase Phosphatase-1 in Human Endothelial Cells via Rac1 and NAD(P)H Oxidase/Nox2-Activation
Circ. Res., January 7, 2005; 96(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Rose, A. E. Lomax, C. S. Kondo, M. B. Anand-Srivastava, and W. R. Giles
Effects of C-type natriuretic peptide on ionic currents in mouse sinoatrial node: a role for the NPR-C receptor
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1970 - H1977.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Rose, A. E. Lomax, and W. R. Giles
Inhibition of L-type Ca2+ current by C-type natriuretic peptide in bullfrog atrial myocytes: an NPR-C-mediated effect
Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2454 - H2462.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. R. Grider
Neurotransmitters Mediating the Intestinal Peristaltic Reflex in the Mouse
J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 460 - 467.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. R. Hirsch, N. Skutta, and E. Schlatter
Signaling and distribution of NPR-Bi, the human splice form of the natriuretic peptide receptor type B
Am J Physiol Renal Physiol, August 1, 2003; 285(2): F370 - F374.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Zhou and K. S. Murthy
Identification of the G protein-activating sequence of the single-transmembrane natriuretic peptide receptor C (NPR-C)
Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1255 - C1261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Doyle, J. Upshaw-Earley, E. L. Bell, and H. C. Palfrey
Natriuretic peptide receptor-B in adult rat ventricle is predominantly confined to the nonmyocyte population
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2117 - H2123.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Holtwick, M. Gotthardt, B. Skryabin, M. Steinmetz, R. Potthast, B. Zetsche, R. E. Hammer, J. Herz, and M. Kuhn
Smooth muscle-selective deletion of guanylyl cyclase-A prevents the acute but not chronic effects of ANP on blood pressure
PNAS, May 14, 2002; 99(10): 7142 - 7147.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. M. Bryan and L. R. Potter
The Atrial Natriuretic Peptide Receptor (NPR-A/GC-A) Is Dephosphorylated by Distinct Microcystin-sensitive and Magnesium-dependent Protein Phosphatases
J. Biol. Chem., May 3, 2002; 277(18): 16041 - 16047.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Takei and S. Hirose
The natriuretic peptide system in eels: a key endocrine system for euryhalinity?
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R940 - R951.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. H. Rho, W. J. Perkins, R. R. Lorenz, D. O. Warner, and K. A. Jones
Differential effects of soluble and particulate guanylyl cyclase on Ca2+ sensitivity in airway smooth muscle
J Appl Physiol, January 1, 2002; 92(1): 257 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B.-Q. Teng, J. R. Grider, and K. S. Murthy
Identification of a VIP-specific receptor in guinea pig tenia coli
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G718 - G725.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
B. C Kone
Molecular biology of natriuretic peptides and nitric oxide synthases
Cardiovasc Res, August 15, 2001; 51(3): 429 - 441.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
T. Suzuki, T. Yamazaki, and Y. Yazaki
The role of the natriuretic peptides in the cardiovascular system
Cardiovasc Res, August 15, 2001; 51(3): 489 - 494.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. D. Doyle, J. Upshaw-Earley, E. L. Bell, and H. C. Palfrey
Natriuretic peptide receptor-B in adult rat ventricle is predominantly confined to the nonmyocyte population
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2117 - H2123.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online