AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G12-G19, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Courtois, F.
Right arrow Articles by Levy, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Courtois, F.
Right arrow Articles by Levy, E.
Vol. 279, Issue 1, G12-G19, July 2000

Iron-ascorbate alters the efficiency of Caco-2 cells to assemble and secrete lipoproteins

F. Courtois1,2, I. Suc1, C. Garofalo1,2, M. Ledoux2, E. Seidman1,3, and E. Levy1,2

1 Centre de Recherche, Hôpital Sainte-Justine, and Departments of 2 Nutrition and 3 Pediatrics, University of Montreal, Montreal, Quebec, Canada H3T 1C5

Although oxidative stress has been implicated in development of gut pathologies, its role in intestinal fat transport has not been investigated. We assessed the effect of Fe2+-ascorbate-mediated lipid peroxidation on lipid synthesis, apolipoprotein biogenesis, and lipoprotein assembly and secretion. Incubation of postconfluent Caco-2 cells with iron(II)-ascorbate (0.2 mM/2 mM) in the apical compartment significantly promoted malondialdehyde formation without affecting sucrase activity, transepithelial resistance, DNA and protein content, and cell viability. However, addition of the oxygen radical-generating system reduced 1) [14C]oleic acid incorporation into cellular triglycerides (15%, P < 0.0002) and phospholipids (16%, P < 0.0005); 2) de novo synthesis of cellular apolipoprotein A-I (apo A-I) (18%, P < 0.05), apo A-IV (38%, P < 0.05), and apo B-48 (45%, P < 0.003) after [35S]methionine addition; and 3) production of chylomicrons (50%), VLDL (40%), LDL (37%), and HDL (30%) (all P < 0.0001). In contrast, increased total cellular cholesterol formation (96%, P < 0.0001), assayed by [14C]acetate incorporation, was noted, attributable to marked elevation (70%, P < 0.04) in activity of DL-3-hydroxy-3-methyl-glutaryl-CoA reductase, the rate-limiting enzyme in cholesterol synthesis. The ratio of Acyl-CoA to cholesterol acyltransferase, the esterifying cholesterol enzyme, remained unchanged. Fe2+-ascorbate-mediated lipid peroxidation modifies intracellular fat absorption and may decrease enterocyte efficiency in assembling and transporting lipids during gut inflammation.

oxidative stress; intestine; apolipoproteins; fat malabsorption


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Z. Ravid, M. Bendayan, E. Delvin, A. T. Sane, M. Elchebly, J. Lafond, M. Lambert, G. Mailhot, and E. Levy
Modulation of intestinal cholesterol absorption by high glucose levels: impact on cholesterol transporters, regulatory enzymes, and transcription factors
Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G873 - G885.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Montoudis, E. Seidman, F. Boudreau, J.-F. Beaulieu, D. Menard, M. Elchebly, G. Mailhot, A.-T. Sane, M. Lambert, E. Delvin, et al.
Intestinal fatty acid binding protein regulates mitochondrion {beta}-oxidation and cholesterol uptake
J. Lipid Res., May 1, 2008; 49(5): 961 - 972.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
V. Marcil, E. Delvin, A. T. Sane, A. Tremblay, and E. Levy
Oxidative stress influences cholesterol efflux in THP-1 macrophages: Role of ATP-binding cassette A1 and nuclear factors
Cardiovasc Res, December 1, 2006; 72(3): 473 - 482.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. T. Sane, D. Sinnett, E. Delvin, M. Bendayan, V. Marcil, D. Menard, J.-F. Beaulieu, and E. Levy
Localization and role of NPC1L1 in cholesterol absorption in human intestine
J. Lipid Res., October 1, 2006; 47(10): 2112 - 2120.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan
Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine
J. Cell Sci., January 15, 2004; 117(2): 327 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Bernotti, E. Seidman, D. Sinnett, S. Brunet, S. Dionne, E. Delvin, and E. Levy
Inflammatory reaction without endogenous antioxidant response in Caco-2 cells exposed to iron/ascorbate-mediated lipid peroxidation
Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G898 - G906.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
F. Courtois, E. G Seidman, E. Delvin, C. Asselin, S. Bernotti, M. Ledoux, and E. Levy
Membrane peroxidation by lipopolysaccharide and iron-ascorbate adversely affects Caco-2 cell function: beneficial role of butyric acid
Am. J. Clinical Nutrition, March 1, 2003; 77(3): 744 - 750.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
F. Courtois, E. Delvin, M. Ledoux, E. Seidman, J.-C. Lavoie, and E. Levy
The Antioxidant BHT Normalizes Some Oxidative Effects of Iron + Ascorbate on Lipid Metabolism in Caco-2 Cells
J. Nutr., June 1, 2002; 132(6): 1289 - 1292.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online