| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Physiology and Biophysics and 2 Internal Medicine, University of Texas Medical Branch, Galveston, Texas, 77555
The molecular identities
of functional chloride channels in hepatocytes are largely unknown. We
examined the ClC-3 chloride channel in rat hepatocytes and found that
mRNA for two different isoforms is present. A short form is identical
to the previously reported sequence for rat ClC-3, and a long form
contains a 176-bp insertion immediately upstream of the translation
initiation site. This predicts a 58-amino acid NH2 terminal
insertion. Both long and short form mRNA was expressed in diverse
tissues of the rat. Transient transfection of the long form in CHO-K1
cells resulted in currents with an I
> B > Cl selectivity sequence, outward
rectification, and inactivation at positive voltages. Short form
currents had identical ionic selectivity but displayed a more extreme
outward rectification and showed no voltage-dependent inactivation.
Immunofluorescence and immunoblots localized native ClC-3
preferentially but not exclusively to the canalicular membrane. We have
therefore identified a new isoform of rat ClC-3 and shown that
expression of both isoforms produces functional channels. In
hepatocytes, ClC-3 is located in association with the canalicular membrane.
ion channels; liver; CHO-K1 cells; canalicular membrane
This article has been cited by other articles:
|
|
 |
|
  Z. Yin, Y. Tong, H. Zhu, and M. A. Watsky ClC-3 is required for LPA-activated Cl- current activity and fibroblast-to-myofibroblast differentiation Am J Physiol Cell Physiol, February 1, 2008; 294(2): C535 - C542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Matsuda, M. S. Filali, K. A. Volk, M. M. Collins, J. G. Moreland, and F. S. Lamb Overexpression of CLC-3 in HEK293T cells yields novel currents that are pH dependent Am J Physiol Cell Physiol, January 1, 2008; 294(1): C251 - C262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Denton, K. Nehrke, X. Yin, A. M. Beld, and K. Strange Altered gating and regulation of a carboxy-terminal ClC channel mutant expressed in the Caenorhabditis elegans oocyte Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1109 - C1118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-Z. Lan, H. Abbas, H. D. Lam, A.-M. Lemay, and C. E. Hill Contribution of a time-dependent and hyperpolarization-activated chloride conductance to currents of resting and hypotonically shocked rat hepatocytes Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G221 - G229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Jin, J. K. Kim, D. K. Yang, S. J. Cho, J. M. Kim, E. J. Koh, H. C. Jung, I. So, and K. W. Kim Fundamental role of ClC-3 in volume-sensitive Cl- channel function and cell volume regulation in AGS cells Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G938 - G948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-X. Wang, W. J. Hatton, G. L. Wang, J. Zhong, I. Yamboliev, D. Duan, and J. R. Hume Functional effects of novel anti-ClC-3 antibodies on native volume-sensitive osmolyte and anion channels in cardiac and smooth muscle cells Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1453 - H1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Olsen, S. Schade, S. A. Lyons, M. D. Amaral, and H. Sontheimer Expression of Voltage-Gated Chloride Channels in Human Glioma Cells J. Neurosci., July 2, 2003; 23(13): 5572 - 5582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gentzsch, L. Cui, A. Mengos, X.-b. Chang, J.-H. Chen, and J. R. Riordan The PDZ-binding Chloride Channel ClC-3B Localizes to the Golgi and Associates with Cystic Fibrosis Transmembrane Conductance Regulator-interacting PDZ Proteins J. Biol. Chem., February 14, 2003; 278(8): 6440 - 6449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Isnard-Bagnis, N. Da Silva, V. Beaulieu, A. S. L. Yu, D. Brown, and S. Breton Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM Am J Physiol Cell Physiol, January 1, 2003; 284(1): C220 - C232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mohammad-Panah, C. Ackerley, J. Rommens, M. Choudhury, Y. Wang, and C. E. Bear The Chloride Channel ClC-4 Co-localizes with Cystic Fibrosis Transmembrane Conductance Regulator and May Mediate Chloride Flux across the Apical Membrane of Intestinal Epithelia J. Biol. Chem., January 4, 2002; 277(1): 566 - 574. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. S. Lamb, R. W. Graeff, G. H. Clayton, R. L. Smith, B. C. Schutte, and P. B. McCray Jr. Ontogeny of CLCN3 Chloride Channel Gene Expression in Human Pulmonary Epithelium Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 376 - 381. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Roman, R. L. Smith, A. P. Feranchak, G. H. Clayton, R. B. Doctor, and J. G. Fitz ClC-2 chloride channels contribute to HTC cell volume homeostasis Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G344 - G353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Huang, J. Liu, A. Di, N. C. Robinson, M. W. Musch, M. A. Kaetzel, and D. J. Nelson Regulation of Human CLC-3 Channels by Multifunctional Ca2+/Calmodulin-dependent Protein Kinase J. Biol. Chem., June 1, 2001; 276(23): 20093 - 20100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, T. Wang, Z. Zhao, and S. A. Weinman The ClC-3 chloride channel promotes acidification of lysosomes in CHO-K1 and Huh-7 cells Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1483 - C1491. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
