AJP - GI Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G277-G287, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mignen, O.
Right arrow Articles by Harvey, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mignen, O.
Right arrow Articles by Harvey, B. J.
Vol. 279, Issue 2, G277-G287, August 2000

Basolateral outward rectifier chloride channel in isolated crypts of mouse colon

Olivier Mignen1, Stéphane Egee1, Martine Liberge2, and Brian J. Harvey3

1 Centre National de la Recherche Scientifique, Unité de Recherche en Physiologie Cellulaire, Université de Bretagne Occidentale, 29200 Brest, France; 2 Laboratoire de Biologie et Physiologie Animales, Université des Antilles et de la Guyane, Campus Fouillote, 97159 Pointe à Pitre, Guadeloupe; and 3 Cellular Physiology Research Unit, University College Cork, Cork, Ireland

Single channel patch-clamp techniques were used to demonstrate the presence of outwardly rectifying chloride channels in the basolateral membrane of crypt cells from mouse distal colon. These channels were rarely observed in the cell-attached mode and, in the inside-out configuration, only became active after a delay and depolarizing voltage steps. Single channel conductance was 23.4 pS between -100 and -40 mV and increased to 90.2 pS between 40 and 100 mV. The channel permeability sequence for anions was: I- > SCN- > Br- > Cl- > NO3- > F- SO42- approx  gluconate. In inside-out patches, the channel open probability was voltage dependent but insensitive to intracellular Ca2+ concentration. In cell-attached mode, forskolin, histamine, carbachol, A-23187, and activators of protein kinase C all failed to activate the channel, and activity could not be evoked in inside-out patches by exposure to the purified catalytic subunit of cAMP-dependent protein kinase A. The channel was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoate, 9-anthracenecarboxylic acid, and DIDS. Stimulation of G proteins with guanosine 5'-O-(3-thiotriphosphate) decreased the channel open probability and conductance, whereas subsequent addition of guanosine 5'-O-(2-thiodiphosphate) reactivated the channel.

ionic channels; colonic crypts; basolateral membrane; mouse; G protein


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Fischer, B. Illek, W. E. Finkbeiner, and J. H. Widdicombe
Basolateral Cl channels in primary airway epithelial cultures
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1432 - L1443.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Li, S. T. Halm, and D. R. Halm
Secretory activation of basolateral membrane Cl- channels in guinea pig distal colonic crypts
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C918 - C933.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Catalan, I. Cornejo, C. D. Figueroa, M. I. Niemeyer, F. V. Sepulveda, and L. P. Cid
ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization
Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G1004 - G1013.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online