AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 279: G400-G410, 2000;
0193-1857/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hagen, S. J.
Right arrow Articles by Morrison, S. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hagen, S. J.
Right arrow Articles by Morrison, S. W.
Vol. 279, Issue 2, G400-G410, August 2000

NH4Cl inhibition of acid secretion: possible involvement of an apical K+ channel in bullfrog oxyntic cells

Susan J. Hagen, Hui Wu, and Sarah W. Morrison

Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215

This study was undertaken to determine the mechanism by which ammonium chloride (NH4Cl) inhibits stimulated acid secretion in the bullfrog gastric mucosa. To this end, four possible pathways of inhibition were studied: 1) blockade of basolateral K+ channel, 2) blockade of ion transport activity, 3) neutralization of secreted H+ in the luminal solution, or 4) ATP depletion. Addition of nutrient 10 mM NH4Cl (calculated NH3 concentration = 92.5 µM and NH4+ concentration = 9.91 mM) inhibited acid secretion within 30 min. Inhibition of acid secretion did not occur by blockade of basolateral K+ channel activity or ion transport activity or by neutralization of the luminal solution. Although ATP depletion occurred in the presence of NH4Cl, the magnitude of ATP depletion in 30 min was not sufficient to inhibit stimulated acid secretion. By comparing the effect of NH4Cl on the resistance of inhibited or stimulated tissues, we demonstrate that NH4Cl acts specifically on stimulated tissues. We propose that NH4Cl blocks activity of an apical K+ channel present in stimulated oxyntic cells. Our data suggest that the activity of this channel is important for the regulation of acid secretion in bullfrog oxyntic cells.

Rana catesbeiana; gastric mucosa; ammonia


This article has been cited by other articles:


Home page
 MicrobiologyHome page
S. D. Lytton, W. Fischer, W. Nagel, R. Haas, and F. X. Beck
Production of ammonium by Helicobacter pylori mediates occludin processing and disruption of tight junctions in Caco-2 cells
Microbiology, October 1, 2005; 151(10): 3267 - 3276.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. McDaniel, A. J. Pace, S. Spiegel, R. Engelhardt, B. H. Koller, U. Seidler, and C. Lytle
Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogen
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G550 - G560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. Abdoun, F. Stumpff, K. Wolf, and H. Martens
Modulation of electroneutral Na transport in sheep rumen epithelium by luminal ammonia
Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G508 - G520.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Xu, J. Henriksnas, S. Barone, D. Witte, G. E. Shull, J. G. Forte, L. Holm, and M. Soleimani
SLC26A9 is expressed in gastric surface epithelial cells, mediates Cl-/HCO3- exchange, and is inhibited by NH4+ (Report)
Am J Physiol Cell Physiol, August 1, 2005; 289(2): C493 - C505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Danzer, M. Jocic, C. Samberger, E. Painsipp, E. Bock, M.-A. Pabst, K. Crailsheim, R. Schicho, I. T. Lippe, and P. Holzer
Stomach-brain communication by vagal afferents in response to luminal acid backdiffusion, gastrin, and gastric acid secretion
Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G403 - G411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. Nakamura and S. J. Hagen
Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1264 - G1275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. M. Cheng, S. W. Morrison, D. X. Yang, and S. J. Hagen
Energy dependence of restitution in the gastric mucosa
Am J Physiol Cell Physiol, August 1, 2001; 281(2): C430 - C438.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online